Homogeneity and the electrophoretic behavior of some proteins; reversible spreading and steady-state boundary criteria.
Homogeneity and the electrophoretic behavior of some proteins; reversible spreading and steady-state boundary criteria.
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一些蛋白质的均一性和电泳行为；
DOI：
10.1021/j150464a007
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发表时间：
1948
期刊：
影响因子：
--
通讯作者：
R. Alberty
中科院分区：
文献类型：
--
作者：
E. A. Anderson;R. Alberty
在蛋白质的平均等电点和低蛋白质浓度下进行的电泳实验中，使得电场和 pH 值通过蛋白质边界基本恒定，可逆扩散表明电泳不均匀性。在这些条件下，Alberty (1) 表明，在高斯迁移率分布的情况下，迁移率分布的标准偏差可以根据蛋白质边界的纹影照片来确定。在实验过程中蛋白质存在可测量的扩散并且迁移率分布可以用高斯概率函数表示的情况下，实验折射率梯度将具有高斯形式，并且迁移率分布的标准偏差h（异质性常数）可以使用公式1计算
In electrophoretic experiments carried out at the average isoelectric point of the protein and at a low protein concentration so that the electric field and pH are essentially constant through the protein boundary, reversible spreading indicates electrophoretic heterogeneity. Under these conditions, Alberty (1) has shown that in the case of a Gaussian mobility distribution, the standard deviation of the mobility distribution may be determined from the schlieren photographs of the protein boundary. In the case where there is measurable dif-fusion of the protein during the experiment and the mobility distribution may be represented by the Gaussian probability function, the experimental refractive-in-dex gradient will have Gaussian form, and the standard deviation of the mobility distribution, h (heterogeneity constant), may be calculated by using equation 1