Transcriptional profiling of Haemophilus parasuis SH0165 response to tilmicosin.
Transcriptional profiling of Haemophilus parasuis SH0165 response to tilmicosin.
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DOI：
10.1089/mdr.2012.0047
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发表时间：
2012-11
影响因子：
2.6
通讯作者：
Yingyu Liu;Pin Chen;Yang Wang;Wentao Li;Shuang Cheng;Chunmei Wang;A. Zhang;Qigai He
中科院分区：
文献类型：
--
作者：
Yingyu Liu;Pin Chen;Yang Wang;Wentao Li;Shuang Cheng;Chunmei Wang;A. Zhang;Qigai He
副猪嗜血杆菌呼吸道病原体对养猪业构成严重威胁，尽管现有的抗菌治疗。为了更详细地了解副猪嗜血杆菌对tilmicosin反应的分子机制，应用微阵列技术分析了体外亚抑制（0.25 μg/ml）和抑制（8 μg/ml）浓度处理副猪嗜血杆菌SH0165的基因表达变化。Tilmicosin处理诱导了405个基因的差异表达，其编码产物主要参与热休克反应、蛋白质合成和细胞内运输。tilmicosin的亚抑制和抑制浓度分别诱导了不同基因表达谱的共享和独特变化。这些变化包括302个主要涉及蛋白质输出和磷酸转移酶系统以维持细胞生长的基因，198个主要涉及RNA聚合酶、重组和修复以抑制细胞生长的基因。对差异表达基因相关功能的计算机分析表明，副猪嗜血杆菌SH0165对tilmicosin的适应涉及蛋白质合成和膜运输的调节。总的来说，包含副猪嗜血杆菌对替尔米考辛反应的每个转录谱的基因为这种具有经济意义的细菌的生理功能提供了新的见解，并可能代表未来分子治疗策略的目标。
The Haemophilus parasuis respiratory tract pathogen poses a severe threat to the swine industry despite available antimicrobial therapies. To gain a more detailed understanding of the molecular mechanisms underlying H. parasuis response to tilmicosin treatment, microarray technology was applied to analyze the variation in gene expression of isolated H. parasuis SH0165 treated in vitro with subinhibitory (0.25 μg/ml) and inhibitory (8 μg/ml) concentrations. Tilmicosin treatment induced differential expression of 405 genes, the encoded products of which are mainly involved in the heat shock response, protein synthesis, and intracellular transportation. The subinhibitory and inhibitory concentrations of tilmicosin induced distinctive gene expression profiles of shared and unique changes, respectively. These changes included 302 genes mainly involved in protein export and the phosphotransferase system to sustain cell growth, and 198 genes mainly related to RNA polymerase, recombination, and repair to inhibit cell growth. In silico analysis of functions related to the differentially expressed genes suggested that adaptation of H. parasuis SH0165 to tilmicosin involves modulation of protein synthesis and membrane transport. Collectively, the genes comprising each transcriptional profile of H. parasuis response to tilmicosin provide novel insights into the physiological functions of this economically significant bacterium and may represent targets of future molecular therapeutic strategies.