Advances in the development and application of microbial consortia for metabolic engineering.
Advances in the development and application of microbial consortia for metabolic engineering.
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DOI：
10.1016/j.mec.2019.e00095
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发表时间：
2019-12-01
影响因子：
5.2
通讯作者：
Koffas, Mattheos Ag
中科院分区：
文献类型：
--
作者：
Jawed, Kamran;Yazdani, Syed Shams;Koffas, Mattheos Ag
代谢工程的最新进展使得能够通过在单一微生物宿主中表达复杂的生物合成途径来生产高价值化学品。然而，许多工程菌株由于氧化还原失衡和代谢负担过重而导致产物产量不佳，并且需要对途径进行划分以实现最佳功能。为了解决这个问题，在共培养多个工程微生物菌株方面取得了重大进展，以在共培养伙伴之间分配代谢负担并提高产品产量。在这种新兴方法中，代谢途径模块可以在合适的宿主中单独优化，然后将它们组合起来以实现完整途径的最佳功能。这种模块化方法拓宽了微调复杂生产平台的可能性，从而实现非常复杂化合物的生物合成。在这里，我们回顾了天然和人工共培养系统在代谢工程中的不同应用和整体潜力，以改善生物生产/生物转化。除了相对于单一栽培的几个优势之外，本综述还讨论了与共培养相关的主要挑战和机遇。
Recent advances in metabolic engineering enable the production of high-value chemicals via expressing complex biosynthetic pathways in a single microbial host. However, many engineered strains suffer from poor product yields due to redox imbalance and excess metabolic burden, and require compartmentalization of the pathway for optimal function. To address this problem, significant developments have been made towards co-cultivation of more than one engineered microbial strains to distribute metabolic burden between the co-cultivation partners and improve the product yield. In this emerging approach, metabolic pathway modules can be optimized separately in suitable hosts that will then be combined to enable optimal functionality of the complete pathway. This modular approach broadens the possibilities to fine tune sophisticated production platforms and thus achieve the biosynthesis of very complex compounds. Here, we review the different applications and the overall potential of natural and artificial co-cultivation systems in metabolic engineering in order to improve bioproduction/bioconversion. In addition to the several advantages over monocultures, major challenges and opportunities associated with co-cultivation are also discussed in this review.