COLLOIDAL GOLD, A USEFUL MARKER FOR TRANSMISSION AND SCANNING ELECTRON-MICROSCOPY
COLLOIDAL GOLD, A USEFUL MARKER FOR TRANSMISSION AND SCANNING ELECTRON-MICROSCOPY
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DOI：
10.1177/25.4.323352
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发表时间：
1977-01-01
影响因子：
3.2
通讯作者：
ROSSET, J
中科院分区：
文献类型：
--
作者：
HORISBERGER, M;ROSSET, J
用单层特定大分子标记的金颗粒制备了尺寸适合透射电子显微镜 [TEM] 和扫描电子显微镜 [SEM] 的电子致密标记物。确定了制备标记物的最佳条件。该方法简单、快速且具有通用性，因为金颗粒已被多糖和蛋白质标记。由于可以制备不同尺寸的均匀金颗粒群，因此该方法也适用于双标记实验。 Au 技术通过 TEM 定位酵母 [Candida utilis Saccharomyces cerevisiae] 细胞壁和 [人] 红细胞膜上的多糖和糖蛋白，以及通过 SEM 定位酵母细胞和完整红细胞上的多糖和糖蛋白来说明。在所有情况下都实现了标记的良好空间分辨率。该方法也适用于标记薄切片。使用分光光度测量来确定每个细胞吸附的金颗粒的数量。
Electron dense markers of a size suitable for transmission electron microscopy [TEM] and scanning electron microscopy [SEM] were prepared with Au granules labeled with a monolayer of specific macromolecules. The optimum conditions for preparing the markers were ascertained. The method is simple, rapid and seems to be general since Au granules have been labeled with polysaccharides and proteins. As homogeneous populations of Au granules having different sizes can be prepared, the method is also suitable for double marking experiments. The Au technique is illustrated by the localization of polysaccharides and glycoproteins on yeast [Candida utilis Saccharomyces cerevisiae] cell walls and [human] erythrocyte membranes by TEM and on yeast cells and intact erythrocytes by SEM. Good spatial resolution of the marker was achieved in all cases. The method is also suitable for marking thin sections. Spectrophotometric measurements were used to determine the number of Au granules adsorbed per cell.