Freeze-fracture organization of hair cell synapses in the sensory epithelium of guinea pig organ of Corti.
Freeze-fracture organization of hair cell synapses in the sensory epithelium of guinea pig organ of Corti.
复制标题
豚鼠柯蒂氏器感觉上皮毛细胞突触的冷冻断裂组织。
DOI：
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Kogaku Saito
中科院分区：
文献类型：
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作者：
Kogaku Saito
用冷冻断裂电镜观察了豚鼠Corti器感觉上皮内传入和传出毛细胞突触的微细结构。在传入突触中，在位于突触前膜活动区的突触前致密突起的正下方的传入突触前膜的P面上看到直径约10 nm的棒状膜内颗粒（IMPs）聚集体。在突触前膜上可以看到大大小小的凹陷。前者在棒状聚集体附近观察到，而后者在距离聚集体一定距离处观察到。在外毛细胞中，在传入突触后膜的P面上观察到直径约10 nm的IMP，密度为3，000/μ m 2。在传出突触中，在突触前膜上观察到由数个至数十个直径为13 nm的大IMP组成的许多聚集体。这些聚集体被定位到小的膜凹陷，这往往是更深的颗粒数每个聚集体增加。在传出突触后膜的P面上观察到直径约为9 nm的密集IMP群，密度为4，000/μ m 2。有孔的突触下池完全覆盖传出突触后膜。此外，当传出突触聚集在一起时，突触下池跨越几个传出突触后膜。在毛细胞的传入和传出突触中，突触膜的专门化由IMP特征的显著聚集体表示。在传出突触中，IMP在突触膜内的运动被认为与突触囊泡膜的回收有关。揭示了突触下池与传出突触后膜之间的结构关系。
The fine structure of both the afferent and efferent hair cell synapses in the sensory epithelium of guinea pig organ of Corti was examined by freeze-fracture electron microscopy. In the afferent synapse, barlike aggregates of intramembrane particles (IMPs) of about 10 nm in diameter were seen on the P-face of the afferent presynaptic membrane directly beneath the presynaptic dense projection which is located in the active zone of the presynaptic membrane. Small and large depressions have been seen on the presynaptic membrane. The former were observed in the proximity of the barlike aggregates, while the latter were observed some distance from the aggregate. In outer hair cells, IMPs of about 10 nm in diameter were seen on the P-face of the afferent postsynaptic membrane at a density of 3,000/microns 2. In the efferent synapse, many aggregates composed of from several to tens of large IMPs of 13 nm in diameter were observed on the presynaptic membrane. These aggregates were localized to small membrane depressions, which tended to be deeper as particle number per aggregate increased. Dense populations of IMPs of about 9 nm in diameter were observed on the P-face of the efferent postsynaptic membrane at a density of 4,000/microns 2. A fenestrated subsynaptic cistern completely covers the efferent postsynaptic membrane. Moreover, the subsynaptic cistern spans several efferent postsynaptic membranes when efferent synapses are gathered in a group. In the afferent and efferent synapses of hair cells, specializations of the synaptic membranes were represented by marked aggregates characteristic of IMPs. In the efferent synapse, IMP movement inside the synaptic membrane was proposed in relationship to retrival of synaptic vesicle membrane. Structural relationship between the subsynaptic cistern and efferent postsynaptic membrane was revealed.
鸡视网膜光感受器中的突触膜域：薄片和冷冻断裂研究。
DOI：
10.1016/s0022-5320(83)90051-5
发表时间：
1983
期刊：
Journal of ultrastructure research
影响因子：
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作者：
Cooper,NG;McLaughlin,BJ;Boykins,LG
通讯作者：
Boykins,LG