A TRYPSIN INHIBITOR IN THE VISCERA OF JAPANESE COMMON SQUID (TODARODES PACIFICUS) ELICITS INSULINOTROPIC EFFECTS IN DIABETIC GK RATS
A TRYPSIN INHIBITOR IN THE VISCERA OF JAPANESE COMMON SQUID (TODARODES PACIFICUS) ELICITS INSULINOTROPIC EFFECTS IN DIABETIC GK RATS
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DOI：
10.1111/j.1745-4514.2010.00515.x
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发表时间：
2012-02-01
影响因子：
4
通讯作者：
Saeki, Hiroki
中科院分区：
文献类型：
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作者：
Kishimura, Hideki;Fukumorita, Kana;Saeki, Hiroki
研究人员检查了日本鱿鱼（Todarodes pacificus）内脏中存在的胰蛋白酶抑制剂（TI）用于治疗糖尿病的潜在用途。在单糖耐量试验中，粗 TI (C-TI) 级分显着刺激正常 Wistar 大鼠和糖尿病 GK 大鼠的胰岛素分泌。高度纯化的 TI (H-TI) 级分对 GK 大鼠的葡萄糖耐量测试显示出相同的效果。体外胃蛋白酶或胰凝乳蛋白酶消化不会降低C-TI的TI活性，并且C-TI级分不具有α-葡萄糖苷酶抑制活性。这些结果表明，对大鼠胰岛素分泌具有刺激作用的物质是鱿鱼TI，并且对GK大鼠的糖耐量的降血糖作用与鱿鱼TI改善胰岛素分泌有关。
The potential use of trypsin inhibitors (TI) present in the viscera of Japanese common squid (Todarodes pacificus) was examined for diabetes treatment. In a single-glucose tolerance test, the crude TI (C-TI) fraction significantly stimulated insulin secretion by normal Wistar rats and by diabetic GK rats. The highly purified TI (H-TI) fraction displayed the same effect on the glucose tolerance test with the GK rats. The TI activity of C-TI was not decreased by pepsin or chymotrypsin digestion in vitro, and the C-TI fraction did not have a-glucosidase inhibitory activity. These results indicate that the substance with the stimulating effect on insulin secretion in rats is the squid TI and that the hypoglycemic effect on glucose tolerance in GK rats is related to an improvement of insulin secretion due to the squid TI.