Hepatic distribution of iron, copper, zinc and cadmium-containing proteins in normal and iron overload mice
Hepatic distribution of iron, copper, zinc and cadmium-containing proteins in normal and iron overload mice
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DOI：
10.1007/s10534-008-9161-8
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发表时间：
2009-04
期刊：
影响因子：
3.5
通讯作者：
Yan Zhang;Bai Li;Chunying Chen;Zhonghong Gao
中科院分区：
文献类型：
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作者：
Yan Zhang;Bai Li;Chunying Chen;Zhonghong Gao
采用体积排阻高效液相色谱-紫外在线联用技术和电感耦合等离子体质谱法测定了铁超载小鼠肝脏中Fe、Cu、Zn和Cd金属蛋白的亚细胞分布。碰撞池技术用于消除某些元素（如Fe）的多原子干扰。在40分钟的保留时间内获得元素级分的比较分子量（MW）信息。铁只存在于高分子量（HMW）蛋白;铜，锌和镉被发现在不同的MW蛋白。还观察到，研究的这四种元素显示出与HMW组分的主要关联。与正常对照组相比，除Cu外，其余元素含量均显著升高，Cd等元素的分布也发生了变化。这意味着体内铁的过量积累可能会影响锌、镉等元素的代谢。
Subcellular distribution of metal-containing proteins of Fe, Cu, Zn and Cd were determined in the liver samples of iron overload mice by size exclusion high performance liquid chromatography with on-line coupling to UV and inductively coupled plasma mass spectrometry. Collision cell techniques was used to remove polyatomic interferences for some elements, such as Fe. Comparative molecular weight (MW) information of the elemental fraction was obtained within a retention time of 40 min. Fe was present only in high-MW (HMW) protein; Cu, Zn and Cd were found in different MW proteins. It was also observed that these four elements studied showed predominant association with HMW fractions. Moreover, compared with the normal group, we found that the contents of these elements except Cu significantly increased and the distribution of some elements like Cd changed in iron overload mouse liver. It means that excessive iron accumulation in vivo may affect the metabolism of other element such as Zn and Cd.