Variation from cytopathogenic biotype to non-cytopathogenic biotype is correlated with the deletion of cellular sequence from bovine viral diarrhea viruses.
Variation from cytopathogenic biotype to non-cytopathogenic biotype is correlated with the deletion of cellular sequence from bovine viral diarrhea viruses.
复制标题
从致细胞病变生物型到非致细胞病变生物型的变异与牛病毒性腹泻病毒的细胞序列删除相关。
DOI：
10.1292/jvms.59.361
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
A. Fukusho
中科院分区：
文献类型：
--
作者：
S. Nakamura;K. Sakamoto;Y. Sakoda;T. Shimazaki;Y. Inoue;N. Ogawa;A. Fukusho
牛病毒性腹泻（BVD）病毒的非致细胞病变（NCP）病毒在对致细胞病变（CP）BVD病毒进行多次空斑克隆后，通过反向空斑形成方法从病毒样本中检测到低比例的非致细胞病变（NCP）病毒； NADL 和 Osloss 菌株。这一现象表明，在CP BVD病毒体外繁殖过程中，NCP BVD病毒的产生率较低。为了研究亲本 CP BVD 病毒和 NCP BVD 病毒作为真实后代的差异，通过 RT-PCR 扩增 NADL 和 Osloss 毒株 p125 结构域中细胞 mRNA 插入的侧翼区域，并将其克隆到 pGEM 3Z 质粒载体中，然后进行测序。因此，证实CP BVD病毒、NADL和Osloss毒株的细胞mRNA插入序列从作为CP BVD病毒、NADL和Osloss毒株的真正后代的NCP BVD病毒中被完全且准确地删除。这些结果表明，NCP BVD 病毒可能通过删除 CP BVD 病毒（NADL 和 Osloss 株）病毒基因组中的细胞 mRNA 插入，从 CP 生物型恢复为 NCP 生物型。
Non-cytopathogenic (NCP) viruses of bovine viral diarrhea (BVD) virus were detected at a low ratio by the reverse plaque formation method from virus samples after several plaque clonings of cytopathogenic (CP) BVD viruses; NADL and Osloss strains. This phenomenon suggests that the NCP BVD viruses are produced at a low ratio during the propagation of CP BVD viruses in vitro. To investigate the differences between the parent CP BVD virus and the NCP BVD virus as a real progeny, the regions flanking the insertion of cellular mRNA in the p125 domain of NADL and Osloss strains were amplified by RT-PCR and cloned into pGEM 3Z plasmid vector, and then sequenced. Consequently, it was confirmed that sequences of cellular mRNA insertion of CP BVD viruses, NADL and Osloss strains, were completely and exactly deleted from the NCP BVD viruses which were real progeny of CP BVD viruses, NADL and Osloss strains. These results suggest that NCP BVD viruses may revert from CP biotype to NCP biotype by the deletion of cellular mRNA insertion in the viral genome of CP BVD viruses (NADL and Osloss strains).