Characterization of Burkholderia pseudomallei and Burkholderia pseudomallei-like strains
Characterization of Burkholderia pseudomallei and Burkholderia pseudomallei-like strains
复制标题
DOI：
10.1017/s095026889600739x
复制
发表时间：
1997-04-01
影响因子：
4.2
通讯作者：
Woods, DE
中科院分区：
文献类型：
--
作者：
Brett, PJ;Deshazer, D;Woods, DE
以前的文献报道表明，假马利氏伯克氏菌菌株可以根据动物毒力进行区分。研究了20株环境源性和临床源性假马勒伯克霍尔德菌分离株对叙利亚金仓鼠外酶的产生、形态和生化表型以及毒力的影响。还测定了这些菌株的16S核糖体RNA （rRNA）基因的部分序列。基于这些观察结果，我们认为高毒力的伪伯克氏菌菌株是真正的伪伯克氏菌菌株。伪伯克霍尔德氏菌真菌株的16S rRNA基因序列与已发表的假伯克霍尔德氏菌序列完全相同，但与低毒菌株的序列存在差异。因此，由于它们在外泌酶产生、仓鼠毒力和16S rRNA基因序列上表现出显著差异，后一种菌株被指定为伪马尔样伯克氏菌。
Previous reports in the literature suggest that Burkholderia pseudomallei strains can be differentiated on the basis of animal virulence. Twenty environmentally and clinically derived isolates of Burkholderia pseudomallei were examined for the production of exoenzymes, morphological and biochemical phenotypes and virulence for Syrian golden hamsters. The partial sequence of the 16S ribosomal RNA [rRNA] genes from a number of these strains was also determined. Based upon these observations, it is suggested that highly virulent Burkholderia pseudomallei strains are true Burkholderia pseudomallei strains. The DNA sequences of the 16S rRNA genes of the true Burkholderia pseudomallei strains were identical to the published sequences for Burkholderia pseudomallei while differences were revealed between the published sequences and those of the lowly virulent strains. Thus, these latter strains have been designated as Burkholderia pseudomallei-like organisms since they demonstrate significant differences in exoenzyme production, hamster virulence and 16S rRNA gene sequences.