Knockdown of HOXB8 inhibits tumor growth and metastasis by the inactivation of Wnt/β-catenin signaling pathway in osteosarcoma
Knockdown of HOXB8 inhibits tumor growth and metastasis by the inactivation of Wnt/β-catenin signaling pathway in osteosarcoma
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DOI：
10.1016/j.ejphar.2019.04.004
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发表时间：
2019-07-05
影响因子：
5
通讯作者：
Dou, Dongmei
中科院分区：
文献类型：
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作者：
Guo, Jiankuo;Zhang, Tianlun;Dou, Dongmei
同源异型盒B8（HOXB 8）是HOX家族的成员，在人类肿瘤中表达异常。然而，其在人骨肉瘤（OS）中的表达模式和功能仍不清楚。本研究的目的是检测其在人OS细胞中的表达和生物学作用。我们的研究结果表明HOXB 8在人OS组织和细胞系中高度表达。HOXB 8的敲除可显着抑制体外OS细胞的增殖，并减弱肿瘤异种移植模型中的肿瘤生长。此外，HOXB 8的敲低显著抑制OS细胞的迁移和侵袭。此外，HOXB 8的敲低有效地阻止了OS细胞中Wnt/β-catenin信号通路的激活。总之，本研究的结果表明，敲低HOXB 8可以通过调节Wnt/β-catenin信号通路来抑制OS中的肿瘤发生和转移。因此，HOXB 8可能代表用于治疗OS的新的治疗靶点。
Homeobox B8 (HOXB8) is a member of HOX family and was reported to be dysregulated in human cancers. However, its expression pattern and function in human osteosarcoma (OS) remain unknown. The aim of the current study is to examine its expression and biological roles in human OS cells. Our results showed that HOXB8 was highly expressed in human OS tissues and cell lines. Knockdown of HOXB8 significantly suppressed the proliferation of OS cells in vitro and attenuated the tumor growth in a tumor xenograft model. In addition, knockdown of HOXB8 dramatically repressed the migration and invasion of OS cells. Furthermore, knockdown of HOXB8 efficiently prevented the activation of Wnt/beta-catenin signaling pathway in OS cells. In conclusion, the findings of the present study demonstrated that knockdown of HOXB8 could suppress tumorigenesis and metastasis in OS through regulation of the Wnt/beta-catenin signaling pathway. Thus, HOXB8 may represent a novel therapeutic target for the treatment of OS.