Functional aspects of the human salivary cystatins in the oral environment
Functional aspects of the human salivary cystatins in the oral environment
复制标题
DOI：
10.1111/j.1601-0825.1999.tb00307.x
复制
发表时间：
1999-07-01
期刊：
影响因子：
3.8
通讯作者：
Featherstone, JDB
中科院分区：
文献类型：
--
作者：
Baron, AC;DeCarlo, AA;Featherstone, JDB
目的：研究人唾液半胱氨酸蛋白酶抑制剂S、SA和SN对半胱氨酸蛋白酶的抑制作用，以寻找可能参与牙周组织破坏的体内靶向半胱氨酸蛋白酶。方法：从人颌下舌下唾液中提纯唾液中的半胱氨酸半胱氨酸酶S大体、S小体、SA、SA和SN。用灵敏的荧光分析法检测了各纯化形式的唾液胱抑素对多种半胱氨酸蛋白酶的抑制作用，并确定了pH是否影响它们对半胱氨酸蛋白酶木瓜酶的抑制活性。结果与结论：唾液半胱氨酸氨基转移酶抑制人溶酶体组织蛋白B、H和L，唾液半胱氨酸氨基转移酶抑制人溶酶体组织蛋白酶L。这些蛋白水解酶参与了牙周组织的破坏，这些数据表明唾液中的半胱氨酸蛋白SA和SN参与了体内蛋白降解事件的控制。唾液半胱氨酸蛋白酶抑制剂S不是半胱氨酸蛋白酶的抑制物，提示其主要作用不是半胱氨酸蛋白酶抑制物。然而，S能够结合更多的钙，并比SA或SN更快地结合到CAP上，这表明它在口腔环境中的主要作用可能是参与牙齿的矿物质平衡。
OBJECTIVES: The aim of this study was to investigate the cysteine protease inhibitory properties of the human salivary cystatins S, SA and SN in order to identify potential in vivo target cysteine proteases which may include those involved in periodontal tissue destruction. In addition, the potential role of the salivary cystatins with respect to the tooth mineral balance and pellicle formation was also investigated.METHODS: Salivary cystatins S major, S minor, SA, SA, (a truncated form of SA) and SN were purified from human submandibular sublingual saliva. Sensitive fluorometric assays were used to test the inhibitory action of each purified form of salivary cystatin against a variety of cysteine proteases and to determine whether pH affected their inhibitory activity towards the well-characterized cysteine protease papain. Their potential role in the mineral balance of the tooth was assessed by the measurement of calcium binding and the rate of binding to carbonated apatite (CAP).RESULTS AND CONCLUSIONS: Salivary cystatin SN was found to inhibit the human lysosomal cathepsins B, H and L and salivary cystatin SA was found to inhibit human lysosomal cathepsin L in vitro. These proteases are involved in periodontal tissue destruction and these data suggest that salivary cystatins SA and SN are involved in the control of the proteolytic events in vivo. Salivary cystatin S was not an inhibitor of the cysteine proteases tested suggesting that its primary role is not as a cysteine protease inhibitor. However, S was able to bind more calcium and bind more rapidly to CAP than SA or SN, suggesting that its primary role in the oral environment is likely to be the involvement with the mineral balance of the tooth.