THE MOLECULAR-BASIS FOR THE DIFFERENTIAL TRANSLATION OF TMV RNA IN TOBACCO PROTOPLASTS AND WHEAT-GERM EXTRACTS
THE MOLECULAR-BASIS FOR THE DIFFERENTIAL TRANSLATION OF TMV RNA IN TOBACCO PROTOPLASTS AND WHEAT-GERM EXTRACTS
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DOI：
10.1002/j.1460-2075.1984.tb01934.x
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发表时间：
1984-01-01
期刊：
影响因子：
11.4
通讯作者：
SICKINGER, HD
中科院分区：
文献类型：
--
作者：
BEIER, H;BARCISZEWSKA, M;SICKINGER, HD
Translation of tobacco mosaic virus (TMV) RNA in tobacco protoplasts yields the 17.5-K coat protein, a 126-K protein and a 183-K protein which is generated by an efficient readthrough over the UAG termination codon at the end of the 126-K cistron. In wheat germ extracts, however, only the 5''-proximal 126-K cistron is translated whereas the 183-K readthrough protein is not synthesized. Purification and sequence analysis of the endogenous tyrosine tRAN revealed that the uninfected tobacco plant contains 2 tRNATyr, both with G.PSI.A anticodons which stimulate the UAG readthrough in vitro and presumably in vivo. In contrast, .apprx. 85% of the tRNATyr from wheat germ contains a Q.PSI.A anticodon and .apprx. 15% has a G.PSI.A anticodon. Otherwise the sequences of tRNATyr from wheat germ and tobacco are identical. UAG readthrough and hence synthesis of the 183-K protein is only stimulated by .**GRAPHIC**. and not at all by .**GRAPHIC**. The tRNAsTyr from wheat leaves were also sequenced. This revealed that adult wheat contains .**GRAPHIC**. only.
