Differential expression of the homeobox gene Hox-1.3 in F9 embryonal carcinoma cells.
Differential expression of the homeobox gene Hox-1.3 in F9 embryonal carcinoma cells.
复制标题
F9胚胎癌细胞中同源盒基因Hox-1.3的差异表达。
DOI：
10.1073/pnas.85.15.5587
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发表时间：
1988
影响因子：
11.1
通讯作者：
Linney,E
中科院分区：
文献类型：
--
作者：
Murphy,SP;Garbern,J;Odenwald,WF;Lazzarini,RA;Linney,E
Hox-1.3基因位于小鼠6号染色体上，先前已显示在小鼠胚胎和成年小鼠中表达。在这项研究中，我们研究了稳态水平的Hox-1.3转录在未分化和分化的F9胚胎癌细胞。我们发现，有一个快速增加的Hox-1.3转录F9细胞分化诱导后。在F9细胞分化诱导后16-24小时，主要的1.85-丝氨酸蛋白酶（kb）转录物的水平达到峰值。利用引物延伸技术，将主要1.85-kb转录物的5'端定位到诱导的F9细胞中的两个位点。RNA的细胞分级分离和转移印迹凝胶分析已将一个次要转录物定位于细胞核，而主要转录物和两个额外的次要转录物出现在诱导的F9细胞的细胞核和细胞质中。用未诱导和诱导的F9细胞核进行的核流出实验的结果表明，在用视黄酸诱导F9细胞后，Hox-1.3转录的速率显著增加。
The Hox-1.3 gene is located on mouse chromosome 6 and has been previously shown to be expressed in mouse embryos and adults. In this study, we have examined the steady-state levels of the Hox-1.3 transcripts in undifferentiated and differentiated F9 embryonal carcinoma cells. We find that there is a rapid increase of Hox-1.3 transcripts after differentiation induction of F9 cells. The level of the major 1.85-kilobase (kb) transcript peaks at 16-24 hr after differentiation induction of F9 cells. By using primer extension techniques the 5' ends of the major 1.85-kb transcript have been mapped to two sites in induced F9 cells. Cellular fractionation of RNA and transfer blot gel analysis has localized one minor transcript to the nucleus, whereas the major transcript and two additional minor transcripts appear in the nucleus and the cytoplasm of induced F9 cells. The results of nuclear run-off experiments with uninduced and induced F9 cell nuclei indicate that there is a substantial increase in the rate of Hox-1.3 transcription upon induction of F9 cells with retinoic acid.