A bioluminescent transgenic mouse model: real-time in vivo imaging of antioxidant EC-SOD gene expression and regulation by interferon gamma.
A bioluminescent transgenic mouse model: real-time in vivo imaging of antioxidant EC-SOD gene expression and regulation by interferon gamma.
复制标题
生物发光转基因小鼠模型：抗氧化剂 EC-SOD 基因表达和干扰素 γ 调节的实时体内成像。
DOI：
10.1016/j.gene.2013.07.012
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发表时间：
2013
期刊：
影响因子：
3.5
通讯作者：
Folz,RodneyJ
中科院分区：
文献类型：
--
作者：
Zelko,IgorN;Stepp,MarcusW;Folz,RodneyJ
Extracellular superoxide dismutase (EC-SOD) is the main antioxidant enzyme in the extracellular matrix. We developed transgenic mice to analyze the EC-SOD promoter activity in vivo in real time and to identify the important cis-elements flanking the 5′ region of the murine EC-SOD gene. Using this model, we demonstrated that luciferase reporter activity correlates closely with endogenous EC-SOD expression, although several interesting differences were also observed. Specifically, luciferase activity was detected at the highest levels in testes, aorta and perirenal fat. Reporter expression was regulated by interferon gamma, a finding that is in agreement with published endogenous EC-SOD gene expression studies. Thus, the 5′-flanking region of mouse EC-SOD gene is responsible, at least in part, for cell specific and inducible expression.
