One facile fluorescence strategy for sensitive detection of endonuclease activity using DNA-templated copper nanoclusters as signal indicators
One facile fluorescence strategy for sensitive detection of endonuclease activity using DNA-templated copper nanoclusters as signal indicators
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一种使用 DNA 模板铜纳米簇作为信号指示剂灵敏检测核酸内切酶活性的简便荧光策略
DOI：
10.1016/j.snb.2016.07.083
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Li Baoxin
中科院分区：
文献类型：
--
作者：
Zhao Hengzhi;Dong Jingjing;Zhou Fulin;Li Baoxin
核酸内切酶活性测定在生物学和医学研究中具有重要意义。在此，开发了一种用于检测核酸内切酶活性的简单易行的策略。该策略通过合理设计含有EcoRI酶切位点的poly（AT-TA）双链DNA作为EcoRI酶切底物和铜纳米团簇（CuNCs）的模板。在EcoRI存在下，双链DNA被切割，然后由于缺乏双链DNA模板，引入Cu 2+和抗坏血酸不能形成荧光CuNC。通过监测荧光强度的变化来定量核酸内切酶活性。在优化条件下，荧光强度与EcoRI浓度在0.002 ~ 0.1 U/μL范围内呈良好的线性关系，检出限为0.00087 U/μL。该方法操作简便，在均相溶液中，整个测定过程可在一个试管中完成。
Assay of endonuclease activity plays an important role in biologic and medicinal study. Herein, a simple and facile strategy was developed for detecting endonuclease activity. In this strategy, poly(AT-TA) double-stranded DNA containing restriction cutting site for EcoRI endonuclease was rationally designed and acted as the substrate of EcoRI and the template for copper nanoclusters (CuNCs). In the presence of EcoRI, the double-stranded DNA was cleaved, and then the introduction of Cu2+and ascorbate cannot form the fluorescent CuNCs due to the lack of the double-stranded DNA templates. The endonuclease actvity was quantified by monitoring the change in fluorescence intensity. Under the optimized conditions, the fluorescence change was linear with EcoRI concentration in the range from 0.002 U/μL to 0.1 U/μL with the detection limit of 0.00087 U/μL. This method was easy and convenient to operate in homogeneous solution, and the whole assay process can be completed in a single tube.
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DOI：
10.1016/j.bbrc.2009.08.164
发表时间：
2009-12
影响因子：
3.1
作者：
H. Urata;Chihiro Tamaki;M. Matsuno;S. Wada;M. Akagi
通讯作者：
H. Urata;Chihiro Tamaki;M. Matsuno;S. Wada;M. Akagi
使用金纳米棒灵敏检测核酸内切酶活性和抑制
影响因子：
12.6
作者：
Deng, Jing;Jin, Yan;Zhang, Chengxiao
通讯作者：
Zhang, Chengxiao
影响因子：
7.4
作者：
Fengzhou Xu;Hui Shi;Xiaoxiao He;Kemin Wang;Dinggeng He;Qiuping Guo;Zhihe Qing;Lv’an Yan;
通讯作者：
Fengzhou Xu;Hui Shi;Xiaoxiao He;Kemin Wang;Dinggeng He;Qiuping Guo;Zhihe Qing;Lv’an Yan;
影响因子：
2.9
作者：
ALVES, J;RUTER, T;PINGOUD, A
通讯作者：
PINGOUD, A
影响因子：
16.6
作者：
Xu, Xiaoyang;Han, Min Su;Mirkin, Chad A.
通讯作者：
Mirkin, Chad A.