Antigen-coated poly α-hydroxy acid based microparticles for heterologous prime-boost adenovirus based vaccinations.
Antigen-coated poly α-hydroxy acid based microparticles for heterologous prime-boost adenovirus based vaccinations.
复制标题
抗原包被的聚α-羟基酸基微粒，用于异源初免-加强腺病毒疫苗接种。
DOI：
10.1016/j.biomaterials.2012.12.030
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发表时间：
2013
期刊：
影响因子：
14
通讯作者：
Salem,AliasgerK
中科院分区：
文献类型：
--
作者：
Lemke,CaitlinD;Geary,SeanM;Joshi,VijayaB;Salem,AliasgerK
腺病毒作为癌症疫苗的载体显示出有希望的潜力，然而，当涉及同源初免-加强策略时，它们的高免疫原性可能是有问题的。在本文所述的研究中，我们表明，涉及卵清蛋白（OVA）抗原包被的微粒作为初免，编码OVA的腺病毒（AdOVA）作为加强的异源初免-加强疫苗接种在产生OVA特异性CD 8 + T细胞应答方面与同源AdOVA初免-加强疫苗接种同样有效，这转化为有效的肿瘤保护。当在异源初免-加强疫苗接种中用作初免时，具有不同化学性质的OVA包被的可生物降解的基于聚α-羟基酸的微粒在促进OVA特异性CD 8 +T细胞以及提供针对随后的肿瘤攻击的保护方面是相当的。这些发现对于在初免-加强病毒疫苗接种策略中使用基于聚α-羟基酸的微粒具有良好的效果，该策略旨在更安全，并且可能更有效地产生抗肿瘤免疫。
Adenoviruses show promising potential as vectors for cancer vaccines, however, their high immunogenicity can be problematic when it comes to homologous prime-boost strategies. In the studies presented here we show that heterologous prime-boost vaccinations involving ovalbumin (OVA)-antigen-coated microparticles as a prime, and adenovirus encoding OVA (AdOVA) as a boost, were equally as effective as homologous AdOVA prime-boosts at generating OVA-specific CD8+T-cell responses, which translated into effective tumor protection. OVA-coated biodegradable poly α-hydroxy acid-based microparticles of varying chemistries, when used as primes in heterologous prime-boost vaccinations, were comparable in terms of promoting OVA-specific CD8+T cells as well as providing protection against subsequent tumor challenge. These findings auger well for using poly α-hydroxy acid-based microparticles in prime-boost viral vaccination strategies geared toward the safer, and potentially more efficient, generation of anti-tumor immunity.