Comparative analysis of the drought-responsive transcriptome in soybean lines contrasting for canopy wilting
Comparative analysis of the drought-responsive transcriptome in soybean lines contrasting for canopy wilting
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DOI：
10.1016/j.plantsci.2015.08.017
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发表时间：
2015-11-01
期刊：
影响因子：
5.2
通讯作者：
Nguyen, Henry T.
中科院分区：
文献类型：
--
作者：
Prince, Silvas J.;Joshi, Trupti;Nguyen, Henry T.
干旱胁迫导致主要油料种子作物如大豆[Glycine max（L）Merr]的显著产量损失。很少有大豆品系被鉴定为耐冠层萎蔫的，然而，赋予耐受性的分子机制尚未完全理解。为了了解生物学过程，对两个对比大豆品系：干旱敏感（DS）Pana和干旱耐受（DT）P1567690的叶组织进行了全基因组转录组分析。在干旱和对照条件下的DS和DT系的成对比较检测到1914和670个基因在干旱条件下的表达变化大于两倍。对DT系的成对处理比较和基因富集分析表明，与蛋白质结合、水解酶活性、碳水化合物/脂质代谢、与细胞壁、质外体和叶绿素a/B结合蛋白相关的木葡聚糖内切转糖基酶相关的基因下调。另一方面，与生物胁迫反应、离子结合和转运、氧化还原过程和电子载体活性相关的基因表达上调。基因富集分析检测到UDP葡萄糖醛酸转移酶活性编码基因在PI 567690干旱胁迫条件下差异表达。我们在DT系的水通道蛋白基因中发现了有价值的SNPs变异，这些变异在已知的较慢的冠层萎蔫系中是保守的，这将有助于提高耐旱性的大豆的标记辅助选择。(C)2015爱思唯尔爱尔兰有限公司版权所有。
Drought stress causes significant yield losses in major oil seed crops, such as soybean [Glycine max (L) Merr]. Few soybean lines have been identified as canopy-wilting tolerant; however, the molecular mechanism conferring tolerance is not fully understood. To understand the biological process, a whole genome transcriptome analysis was performed for leaf tissues of two contrasting soybean lines: drought-susceptible (DS) Pana and drought-tolerant (DT) P1567690. A pairwise comparison of the DS and DT lines under drought and control conditions detected 1914 and 670 genes with a greater than two-fold change in expression under drought conditions. Pairwise treatment comparison and gene enrichment analysis on the DT line showed the down-regulation of genes associated with protein binding, hydrolase activity, carbohydrate/lipid metabolism, xyloglucan endo-transglycosylases associated with cell-wall, apoplast, and chlorophyll a/b binding proteins. On the other hand, genes that were associated with the biotic stress response, ion binding and transport, the oxido-reductive process and electron carrier activity were up-regulated. Gene enrichment analysis detected UDP glucuronosyl transferase activity-encoding genes to be differentially expressed in PI 567690 under drought stress conditions. We found valuable SNPs variation in aquaporin genes of the DT line that are conserved in known slower canopy-wilting lines, this should facilitate marker-assisted selection in soybeans with improved drought tolerance. (C) 2015 Elsevier Ireland Ltd. All rights reserved.