Co-Encapsulation of EGCG and Quercetin in Liposomes for Optimum Antioxidant Activity
Co-Encapsulation of EGCG and Quercetin in Liposomes for Optimum Antioxidant Activity
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DOI：
10.1111/1750-3841.14405
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发表时间：
2019-01-01
影响因子：
3.9
通讯作者：
Liu, Donghong
中科院分区：
文献类型：
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作者：
Chen, Weijun;Zou, Mingming;Liu, Donghong
尽管已经开发了不同的递送系统来克服表没食子儿茶素-3-没食子酸酯（表没食子儿茶素）和槲皮素在食品应用中的限制，但没有人提到它们的同时封装。在这项研究中，这两种多酚成功地共同载入脂质体。在最佳工艺条件下（卵磷脂：总多酚= 25：1，卵磷脂：胆固醇= 6：1，卵磷脂：吐温80 = 8：1，超声时间2 min），脂质体的平均粒径为111.10 ± 0.52nm，多分散指数为0.259 ± 0.006，Zeta电位为-19.83 ± 0.45mV，包封率分别为64.05 ± 1.56%和61.73 ± 2.55%。储存30天后，观察到平均尺寸增加4.05%，而PDI和zeta电位无显著变化（P >= 0.05）。此外，2，2-二苯基-1-苦基肼（DPPH）法显示了两种化合物在脂质体系统中的协同抗氧化作用。这些结果表明，EGCG和槲皮素的共包封增强了它们的有效性。
Although different delivery systems have been developed to overcome the limits of Epigallocatechin-3-gallate (EGCG) and quercetin in food application, none have referred to their simultaneous encapsulation. In this study, these two polyphenols were successfully co-loaded into liposomes. Under the optimal conditions (lecithin-total polyphenols ratio 25:1, lecithin-cholesterol ratio 6:1, lecithin-Tween 80 ratio 8:1 and ultrasonic time 2 min), the mean size, polydispersity index (PDI) and zeta potential of liposomes were 111.10 +/- 0.52 nm, 0.259 +/- 0.006 and -19.83 +/- 0.45 mV, with an encapsulation efficiency of 64.05 +/- 1.56% and 61.73 +/- 2.55% for EGCG and quercetin, respectively. After 30-day storage, an increase of 4.05% was observed in the mean size with no significant change (P >= 0.05) in the PDI and zeta potential. Moreover, 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay revealed a synergistic antioxidant effect of the two compounds in liposomal system. These results demonstrated that co-encapsulation of EGCG and quercetin enhances their effectiveness.