Different biotinylation strategies for competitive immunoassay of estradiol
Different biotinylation strategies for competitive immunoassay of estradiol
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DOI：
10.1002/cjoc.20040220719
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发表时间：
2010-08
影响因子：
5.4
通讯作者：
Jin-Fu Zhao;Yong-Cheng Wang;Yuan-zong Li;Wen-bao Chang
中科院分区：
文献类型：
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作者：
Jin-Fu Zhao;Yong-Cheng Wang;Yuan-zong Li;Wen-bao Chang
对雌二醇(EZ)的生物素化竞争免疫分析方法进行了研究。建立并优化了两种竞争酶免疫分析(WIA)的生物素-亲和素扩增体系。在一个实验中，仅使用E2-生物素结合物作为示踪剂，在另一个实验中，使用生物素标记抗体作为示踪剂。在两个EIA中，都使用了辣根过氧化物酶标记的亲和素(Avidin-HRP)，并用分光光度法测定了酶的活性。测定并比较其精密度、灵敏度和特异度。结果表明，虽然两种方法的特异性均令人满意，但半抗原生物素的EL4在敏感性和精密度上优于生物素标记的EL4。以E_2-生物素和生物素标记抗体为示踪剂，血清E_2的检出限分别为8和50 pg/m L。
Study on biotinylation strategies for competitive immunoassay of estradiol (Ez) was carried out. Two types of competitive enzyme immunoassay (WIA) with Biotin-Avidin amplification system were established and optimized. The E2-Biotin conjugate was used as a mere in one assay, and biotinylated antibody was used as a tracer in the other. In both of EIAs, horseradish peroxidase-labelled Avidin (Avidin-HRP) was used with a spectrometric determination of enzyme activity. The precision, sensitivity and specificity were measured and compared. The results showed that although both were satisfactory in specificity, the EL4 with hapten-Biotin showed to be superior to the EL4 with biotinylated antibody in sensitivity and precision. The limit of detection of serum E2 was 8 and 50 pg/mL with E2-Biotin and biotinylated antibody as tracer, respectively.