Strenuous running exacerbates knee cartilage erosion induced by low amount of mono-iodoacetate in rats.
Strenuous running exacerbates knee cartilage erosion induced by low amount of mono-iodoacetate in rats.
复制标题
剧烈的跑步加剧了大鼠的单碘乙酸含量少的膝关节软骨侵蚀。
DOI：
10.1186/s12891-017-1393-8
复制
发表时间：
2017-01-25
影响因子：
2.3
通讯作者：
Sekiya I
中科院分区：
文献类型：
--
作者：
Saito R;Muneta T;Ozeki N;Nakagawa Y;Udo M;Yanagisawa K;Tsuji K;Tomita M;Koga H;Sekiya I
炎症阶段的剧烈跑步对大鼠膝关节是否产生有益或有害的影响仍有争议。我们研究了（1）大鼠在30公里跑步过程中的脱落率，（2）剧烈跑步和/或少量单碘乙酸盐注射对软骨的影响，以及（3）剧烈跑步对滑膜炎的影响。强迫大鼠在6周内跑30公里，并检查脱落率。向右膝注射0.1 mg单碘乙酸盐一周后，迫使大鼠在3周内跑15公里或根本不跑，然后评估膝关节软骨。髌下脂肪垫处的滑膜也进行了组织学检查。尽管所有12只老鼠都跑了15公里，但只有6只老鼠跑完了30公里。肉眼观察，0.1 mg单碘乙酸盐引起胫骨软骨侵蚀，无论跑步15 km。在组织学上，0.1 mg单碘乙酸盐诱导胫骨软骨中软骨基质的损失，另外15 km的剧烈跑步显著加剧了损失。单碘乙酸引起的滑膜炎在跑步后得到改善。只有50%的老鼠因为脚部问题完成了30公里的跑步。剧烈跑步进一步加剧了胫骨软骨侵蚀，但不影响单碘乙酸诱导的滑膜炎。
It is still debated whether strenuous running in the inflammatory phase produces beneficial or harmful effect in rat knees. We examined (1) the dropout rate of rats during a 30-km running protocol, (2) influences of strenuous running and/or low amounts of mono-iodoacetate injection on cartilage, and (3) the effect of strenuous running on synovitis. Rats were forced to run 30 km over 6 weeks and the dropout rate was examined. One week after 0.1 mg mono-iodoacetate was injected into the right knee, rats were forced to run either 15 km or not run at all over 3 weeks, after which knee cartilage was evaluated. Synovium at the infrapatellar fat pad was also examined histologically. Even though all 12 rats run up to 15 km, only 6 rats completed 30 km of running. Macroscopically, 0.1 mg mono-iodoacetate induced erosion at the tibial cartilage irrespective of 15 km of running. Histologically, 0.1 mg mono-iodoacetate induced loss of cartilage matrix in the tibial cartilage, and an additional 15 km of strenuous running significantly exacerbated the loss. Synovitis caused by mono-iodoacetate improved after running. Only 50% of rats completed 30 km of running because of foot problems. Strenuous running further exacerbated tibial cartilage erosion but did not influence synovitis induced by mono-iodoacetate.