Regulatory networks revealed by transcriptional profiling of damaged Saccharomyces cerevisiae cells:: Rpn4 links base excision repair with proteasomes
Regulatory networks revealed by transcriptional profiling of damaged Saccharomyces cerevisiae cells:: Rpn4 links base excision repair with proteasomes
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DOI：
10.1128/mcb.20.21.8157-8167.2000
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发表时间：
2000-11-01
影响因子：
5.3
通讯作者：
Samson, LD
中科院分区：
文献类型：
--
作者：
Jelinsky, SA;Estep, P;Samson, LD
暴露于致癌的烷基化剂、氧化剂和电离辐射中，可以调节酿酒酵母6200个基因中超过三分之一的转录水平。计算分析描述了协同调节基因群，其上游区域具有已知的和新的调控序列基序，一组协同调节基因包含许多DNA切除修复基因（包括MAG1 3-甲基腺嘌呤DNA糖基酶基因）和大量蛋白质降解基因。此外，这些基因的转录是由蛋白酶体相关蛋白Rpn4调节的，最有可能是通过其与MAGI上游抑制因子序列2-like元件的结合，结果证明与最近发现的蛋白酶体相关控制元件几乎相同（G. Mannhaupt， R. Schnall， V. Karpov， I. Vetter和H. Feldmann, FEES Lett. 450:27- 34,1999）。我们已经确定了大量转录受Rpn4p影响的基因。
Exposure to carcinogenic alkylating agents, oxidizing agents, and ionizing radiation modulates transcript levels for over one third of Saccharomyces cerevisiae's 6,200 genes. Computational analysis delineates groups of coregulated genes whose upstream regions bear known and novel regulatory sequence motifs, One group of coregulated genes contain a number of DNA excision repair genes (including the MAG1 3-methyladenine DNA glycosylase gene) and a large selection of protein degradation genes. Moreover, transcription of these genes is modulated by the proteasome-associated protein Rpn4, most likely via its binding to MAGI upstream repressor sequence 2-like elements, that turn out to be almost identical to the recently identified proteasome-associated control element (G. Mannhaupt, R. Schnall, V. Karpov, I. Vetter, and H. Feldmann, FEES Lett. 450:27-34, 1999). We have identified a large number of genes whose transcription is influenced by Rpn4p.