Phasically firing neurons in the supraoptic nucleus of the rat hypothalamus: Immunocytochemical and electrophysiological studies
Phasically firing neurons in the supraoptic nucleus of the rat hypothalamus: Immunocytochemical and electrophysiological studies
复制标题
大鼠下丘脑视上核中的阶段性放电神经元：免疫细胞化学和电生理学研究
DOI：
10.1016/0304-3940(83)90509-8
复制
发表时间：
1983
影响因子：
2.5
通讯作者：
Y. Sano
中科院分区：
文献类型：
--
作者：
H. Yamashita;K. Inenaga;M. Kawata;Y. Sano
采用电生理、细胞内荧光染料标记和免疫细胞化学等方法研究了大鼠下丘脑切片后视上神经元放电模式与肽的关系。注射Lucifer Yellow-CH（LY）后，10个大细胞神经元中有7个表现出阶段性放电模式；这些细胞也用抗血管加压素血清染色。本报告提供了直接证据，证明大多数视上核的阶段性放电神经分泌神经元含有抗利尿激素。本研究证明了结合免疫细胞化学和电生理技术研究哺乳动物单个神经元多肽含量的可行性。
Relations between firing patterns and peptides in supraoptic neurons of rat hypothalamic slice preparations were studied by electrophysiology, intracellular fluorescent dye-marking and immunocytochemistry. Seven out of 10 magnocellular neurons which showed phasically firing patterns were identified by injections of Lucifer Yellow-CH(LY); these were also stained with an anti-vasopressin serum. This report presents direct evidence that most of the phasically firing neurosecretory neurons in the supraoptic nucleus contain vasopressin. This study demonstrates the feasibility of combining immunocytochemical and electrophysiological techniques to study the peptides contents of single mammalian neurons.