Metal mesh vitrification (MMV) method for cryopreservation of porcine embryos
Metal mesh vitrification (MMV) method for cryopreservation of porcine embryos
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DOI：
10.1016/j.theriogenology.2008.05.045
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发表时间：
2008-09-15
期刊：
影响因子：
2.8
通讯作者：
Funahashi, H.
中科院分区：
文献类型：
--
作者：
Fujino, Y.;Kojima, T.;Funahashi, H.
目的是开发一种更简单、更可靠的猪胚胎玻璃化冷冻方法。用eCG和hCG诱导初情期前供体母猪排卵，然后进行人工授精。在hCG处理后6或7天收集直径约200 μ m的桑椹胚和扩张囊胚。保存从供体后备母猪中采集的胚胎，以便单独识别，并以4或5个批次进行处理。最小体积的胚胎（
The objective was to develop a simpler, more reliable vitrification method for porcine embryos. Prepubertal donor gilts were induced to ovulate with eCG and hCG, and then inseminated artificially. Morulae and expanding blastocysts approximately 200 mu m in diameter were collected 6 or 7 d after hCG treatment. Embryos collected from donor gilts were maintained, so as to be individually recognizable, and handled in batches of four or five. The embryos together with a minimum volume (