A comparison among HER2, TP53, PAI-1, angiogenesis, and proliferation activity as prognostic variables in tumours from 408 patients diagnosed with early breast cancer
A comparison among HER2, TP53, PAI-1, angiogenesis, and proliferation activity as prognostic variables in tumours from 408 patients diagnosed with early breast cancer
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DOI：
10.1080/02841860801958295
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发表时间：
2008-01-01
期刊：
影响因子：
3.1
通讯作者：
Overgaard, Jens
中科院分区：
文献类型：
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作者：
Offersen, Birgitte Vrou;Alsner, Jan;Overgaard, Jens
背景在408例随访>10年的早期乳腺癌患者的肿瘤中研究了HER 2、TP 53突变、派-1蛋白水平、血管生成和增殖的预后潜力。167例患者（41%）死于乳腺癌。材料和方法。对道岔切片进行HER 2、CD 34和MIB-1染色。HER 2评分基于染色强度，3 +被认为是HER 2+。采用Chalkley法对血管生成进行评分。使用系统随机抽样评价MIB-1。采用ELISA法检测派-1。通过DGGE分析和DNA测序评估TP 53突变。结果91例患者（22%）为HER 2阳性。TP 53突变10 1例（2 5%）。派-1、Chalkley和MB-1的中位数分别为0.72 ng/mg蛋白（范围：0-90 ng/mg蛋白）、5.00（范围：2.67-12.00）和15%（范围：1-83%）。MIB-1与HER 2+、Chalkley计数、TP 53突变相关（均为P
Background. The prognostic potential of HER2, TP53 mutations, PAI-1 protein levels, angiogenesis and proliferation were investigated in tumours from 408 patients with early breast cancer followed >10 years. One hundred and sixty seven patients (41%) died from breast cancer. Materials and methods. Turnout sections were stained for HER2, CD34, and MIB-1. HER2 scores were based on staining intensity, 3 + being considered HER2 +. Angiogenesis was scored by the Chalkley method. MIB-1 was evaluated using systematic random sampling. PAI-1 was measured by ELISA. TP53 mutations were evaluated by DGGE analysis and DNA sequencing. Results. Ninety one patients (22%) were HER2 positive. TP53 was mutated in 10 1 cases (25 %). Median PAI-1, Chalkley and MB-1 was 0.72 ng/mg protein (range, 0-90 ng/mg protein), 5.00 (range, 2.67-12.00) and 15% (range, 1-83%). MIB-1 was correlated with HER2+, Chalkley counts, TP53 mutations (all p