Qualitative and quantitative analysis of chemical constituents of Centipeda minima by HPLC-QTOF-MS & HPLC-DAD
Qualitative and quantitative analysis of chemical constituents of Centipeda minima by HPLC-QTOF-MS & HPLC-DAD
复制标题
HPLC-QTOF-MS 定性定量分析小蜈蚣化学成分
DOI：
10.1016/j.jpba.2016.04.029
复制
发表时间：
2016-06-05
影响因子：
3.4
通讯作者：
Mok, Daniel Kam Wah
中科院分区：
文献类型：
--
作者：
Chan, Chi-on;Jin, Deng-ping;Mok, Daniel Kam Wah
建立了高效液相色谱-四极杆飞行时间质谱（HPLC-QTOF-MS）正、负离子模式分析鹅不食草醇提物（EBSC）的主要成分。通过与外标法保留时间和准确质量的比较，从10批样品中鉴定出黄酮类及其苷类、酚酸类和多酚酸类、倍半萜内酯类等12种常见成分（质量精度在3 ppm以内）或串联MS的裂解模式。同时，建立了准确可靠的HPLC-DAD法，可同时测定10种化学标记物的含量。从方法验证（包括线性、准确度和精密度）中获得的结果表明，该新方法可靠且耐用。异绿原酸A和短梗菊素A在醇提物中含量最高，可作为控制其质量的指标。(C)2016爱思唯尔B. V.保留所有权利。
A high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC-QTOF-MS) method in both positive and negative ion modes was established to investigate the major constituents in the ethanolic extract of Centipeda minima (EBSC). Twelve common components including flavones and their glycosides, phenolic and polyphenolic acids, and sesquiterpene lactone were identified in ten batches of samples based on comparison with the retention time and accurate mass of external standards (mass accuracy within 3 ppm) or the fragmentation patterns of tandem MS. Meanwhile, a simple, accurate and reliable HPLC-DAD method was also developed to determine the content of 10 chemical markers simultaneously. Results obtained from method validations including linearity, accuracy and precision showed that this new method is reliable and robust. Isochlorogenic acid A and brevilin A were found to be the most abundant in the ethanol extract of EBSC and could be served as markers for quality control of EBSC. (C) 2016 Elsevier B.V. All rights reserved.