Visualization of oxygen-concentration-dependent production of nitric oxide in rat hippocampal slices during aglycemia
Visualization of oxygen-concentration-dependent production of nitric oxide in rat hippocampal slices during aglycemia
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DOI：
10.1046/j.1471-4159.2001.00161.x
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发表时间：
2001-03-01
影响因子：
4.7
通讯作者：
Nagano, T
中科院分区：
文献类型：
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作者：
Kojima, H;Hirata, M;Nagano, T
我们的新型荧光指示剂DAF-FM允许活细胞中一氧化氮（NO）的生物成像，在空间和时间上具有高分辨率，在pH 5.8以上具有稳定的强度。一种膜渗透性衍生物，DAF-FM DA，被施加到成像的NO在大鼠海马脑片中产生的暴露于一个glycyrrhiza介质。NO的产生主要集中在CA 1区，并依赖于O-2的浓度。在暴露于缺氧-无糖培养基，NO几乎不产生，而细胞内Ca 2+的显着升高观察。NO的产生急剧增加，只要灌流液被改变为正常的培养基。这些结果表明，NO合酶激活后再灌注，而不是在缺血。
Our novel fluorescent indicator, DAF-FM, permits the bioimaging of nitric oxide (NO) in living cells with high resolution in space and time, with stable intensity above pH 5.8. A membrane-permeable derivative, DAF-FM DA, was applied to imaging of NO generated in rat hippocampal slices by exposure to an aglycemic medium. NO production was observed mainly in the CA1 area, and was dependent on the concentration of O-2. During exposure to an anoxic-aglycemic medium, NO was hardly produced, while marked elevation of intracellular Ca2+ was observed. Production of NO increased sharply as soon as the perfusate was changed to the normal medium. These results suggest that NO synthase is activated after reperfusion rather than during ischemia.