Fatty acid biosynthesis and transcriptional regulation of Stearoyl-CoA Desaturase 1 (SCD1) in buffalo milk
Fatty acid biosynthesis and transcriptional regulation of Stearoyl-CoA Desaturase 1 (SCD1) in buffalo milk
复制标题
水牛乳中脂肪酸生物合成和硬脂酰辅酶A去饱和酶1 (SCD1)的转录调控
DOI：
10.1186/s12863-020-0829-6
复制
发表时间：
2020-03-02
期刊：
影响因子：
2.9
通讯作者：
Liu, Qingyou
中科院分区：
文献类型：
--
作者：
Li, Zhipeng;Lu, Suyu;Liu, Qingyou
水牛奶是一种高营养食品，其脂肪酸含量高，营养成分丰富。布法罗乳脂肪含量较高，适合加工开发各种健康营养产品。此外，布法罗乳含有更多的不饱和脂肪酸（乌法），例如油酸和亚麻酸，由于其理想的生理作用，从人类健康的角度来看，这些脂肪酸是重要的。然而，有关布法罗乳中FA合成的化学组成和机制的信息还不充分。本研究假设SCD 1基因的表达可以改变乳腺上皮细胞FA的生物合成，进而影响布法罗乳中FA的含量。我们调查的转录和生物学作用的硬脂酰辅酶A脱氢酶1（SCD 1）在水牛乳腺上皮细胞（BMEC）在FA和三酰甘油（TAG）synthesization.ResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResultsResult布法罗乳中SCD 1的表达改变后，FAS、ACACA、SREBP 1、PPARG、GPAT和AGPAT基因的表达水平显著增加。此外，BMEC中SCD 1基因的改变也介导了FA生物合成相关基因的表达，从而导致FA组成的改变。SCD 1的过表达显著增加了与FA和TAG合成相关的基因的表达，导致BMEC中FA和不饱和FA含量增加。然而，下调的SCD 1表现出相反的results.ConclusionOur研究提供了机制的见解转录调控SCD 1改变FA和TAG的合成，通过直接或间接介导的生物合成和代谢途径在BMEC。我们提供了关于通过SCD 1信号转导工程化布法罗乳中FA含量的初步发现。
BackgroundBuffalo milk is considered as a highly nutritious food owing to its higher contents of fatty acids (FA) and rich nutrient profile. Higher fat contents of buffalo milk make it suitable for processing to develop various healthy and nutritious products. Moreover, buffalo milk contains more unsaturated FAs (UFA) such as oleic and linolenic acid, which are important from the human health point of view owing to their desirable physiological effects. However, inadequate information is available about the chemical composition and mechanism of FA synthesis in buffalo milk. In this study, we hypothesized that expression ofSCD1 gene could alter the biosynthesis of FA in epithelial cells of mammary gland and subsequently affect the FA contents in buffalo milk. We investigated the transcriptional and biological role of Stearoyl-CoA Desaturase 1 (SCD1) in the buffalo mammary epithelial cells (BMECs) during FA and triacylglycerol (TAG) synthesis.ResultsResults revealed that unsaturated fatty acid contents were much higher in concentration in buffalo milk as compared to Holstein cow. Significant increase in the expression level ofFAS,ACACA,SREBP1,PPARG,GPAT,andAGPATgenes was observed in response to altered expression of SCD1in buffalo milk. Moreover, change inSCD1gene in BMECs also mediated the expression of genes related to FA biosynthesis subsequently leading to alter the FA composition. Overexpression ofSCD1significantly increased the expression of genes associated with FA and TAG synthesis leading to enhance FA and unsaturated FA contents in BMECs. However, down-regulation ofSCD1exhibited opposite consequences.ConclusionOur study provides mechanistic insights on transcriptional regulation ofSCD1 to alter FA and TAG synthesis through directly or indirectly mediating biosynthesis and metabolic pathways in BMECs. We provide preliminary findings regarding engineering of FA contents in buffalo milk throughSCD1 signaling.