In vivo osteogenic durability of cultured bone in porous ceramics: a novel method for autogenous bone graft substitution.
In vivo osteogenic durability of cultured bone in porous ceramics: a novel method for autogenous bone graft substitution.
复制标题
多孔陶瓷中培养骨的体内成骨耐久性：一种自体骨移植替代的新方法。
作者：
T. Yoshikawa;H. Ohgushi;Hiroshi Nakajima;E. Yamada;K. Ichijima;Susumu Tamai;Tomohiro Ohta
背景
当在补充有 15% 胎牛血清、抗坏血酸、β-甘油磷酸和地塞米松的培养基中培养时，骨髓细胞分化为骨形成成骨细胞。
方法
为了研究培养骨髓细胞的体内成骨细胞活性和骨基质形成，将Fischer大鼠骨髓细胞在多孔羟基磷灰石（HA）中培养2周，然后皮下植入7周龄雄性同系大鼠体内。 8周和52周后收获植入物进行生化和组织学分析。
结果
在这两种情况下，在许多HA孔中都观察到板层骨的形成伴随着骨髓的再生。当在植入后 50 周施用荧光染料（钙黄绿素）时，在 52 周收获的植入物的孔中检测到荧光染料。 52 周时，在一些孔中观察到破骨细胞吸收，随后出现新骨形成，表明骨重塑仍在继续。 52周时收获的植入物的碱性磷酸酶活性与8周时相当，而52周时收获的植入物的骨钙素含量约为8周时的两倍。
结论
这些结果表明，预制骨/HA结构具有持续的体内成骨和造血活性，并表明在植入前较脆的结构移植后，正常骨组织得到再生。使用HA和培养的骨髓细胞培养的组织工程可能为骨骼疾病患者提供骨移植的替代方法，尽管还需要进一步的体内和体外实验。
BACKGROUND
Bone marrow cells differentiate into bone-forming osteoblasts when cultured in medium supplemented with 15% fetal bovine serum, ascorbic acid, beta-glycerophosphate, and dexamethasone.
METHODS
To investigate in vivo osteoblastic activity and bone matrix formation by cultured bone marrow cells, Fischer rat marrow cells were cultured for 2 weeks in porous hydroxyapatite (HA) and then subcutaneously implanted into 7-week-old male syngeneic rats. The implants were harvested after 8 and 52 weeks for biochemical and histological analyses.
RESULTS
At both times, formation of lamellar bone accompanied by regeneration of marrow were seen in many of the HA pores. When a fluorochrome (calcein) was administered at 50 weeks after implantation, it was detected in the pores of implants harvested at 52 weeks. Osteoclastic resorption followed by new bone formation was seen in some pores at 52 weeks, indicating that bone remodeling was continuing. The alkaline phosphatase activity of implants harvested at 52 weeks was comparable to that at 8 weeks, whereas the osteocalcin content of the implants harvested at 52 weeks was about twice that at 8 weeks.
CONCLUSION
These results demonstrated that there was persistent in vivo osteogenic and hematopoietic activity in the prefabricated bone/HA constructs, and indicated that normal bone tissue was regenerated after grafting of the constructs, which were brittle before implantation. Tissue engineering using HA and cultured marrow cells culture may provide an alternative method of bone transplantation for patients with skeletal disorders, although further in vivo and in vitro experiments are needed.
影响因子：
4.1
作者：
Bruder, SP;Horowitz, MC;Haynesworth, SE
通讯作者：
Haynesworth, SE