Identification of Arabidopsis ethylene-responsive element binding factors with distinct induction kinetics after pathogen infection
Identification of Arabidopsis ethylene-responsive element binding factors with distinct induction kinetics after pathogen infection
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DOI：
10.1104/pp.010862
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发表时间：
2002-04-01
期刊：
影响因子：
7.4
通讯作者：
Singh, KB
中科院分区：
文献类型：
--
作者：
Oñate-Sánchez, L;Singh, KB
乙烯响应元件结合因子（ERF）蛋白是植物特异性转录因子，其中许多与胁迫反应有关。我们已经鉴定了四个拟南芥 ERF 基因，其表达是由细菌病原体丁香假单胞菌番茄致病变种的无毒和强毒菌株特异性诱导的，具有重叠但不同的诱导动力学。然而，与无毒株相比，强毒株感染后 ERF mRNA 积累延迟。在大多数情况下，ERF 基因表达的诱导先于基本几丁质酶基因的 mRNA 积累，而碱性几丁质酶基因是这些 ERF 中的一个或多个的潜在下游靶标。研究了不同拟南芥组织中 ERF 基因对信号分子乙烯、茉莉酸甲酯和水杨酸 (SA) 的反应，以及在对病原体敏感性降低或增强的拟南芥突变体中的表达，观察到显着差异。例如，在幼苗中，一些ERF基因在野生型中不受SA诱导，但在pad4-1突变体中对SA有反应，这表明PAD4-1在SA积累的上游发挥作用，也参与抑制SA诱导的特定ERF基因的表达。四种 ERF 蛋白均含有转录激活结构域。这些结果表明，涉及 ERF 蛋白的转录激活级联可能对于植物防御病原体攻击很重要，并且一些 ERF 家族成员可能参与 SA 和茉莉酸信号通路之间的串扰。
Ethylene-responsive element binding factors (ERF) proteins are plant-specific transcription factors, many of which have been linked to stress responses. We have identified four Arabidopsis ERF genes whose expression was specifically induced by avirulent and virulent strains of the bacterial pathogen Pseudomonas syringae pv tomato, with overlapping but distinct induction kinetics. However, a delay in ERF mRNA accumulation after infection with the virulent strain was observed when compared with the avirulent strain. The induction of ERF gene expression in most cases preceded the mRNA accumulation of a basic chitinase gene, a potential downstream target for one or more of these ERFs. The expression of the ERF genes was examined among different Arabidopsis tissues, in response to the signaling molecules ethylene, methyl jasmonate, and salicylic acid (SA), and in Arabidopsis mutants with decreased or enhanced susceptibility to pathogens, and significant differences were observed. For example, in seedlings, some of the ERF genes were not induced by SA in the wild-type but were SA responsive in the pad4-1 mutant, suggesting that PAD4-1, which acts upstream of SA accumulation, is also involved in repressing the SA-induced expression of specific ERF genes. The four ERF proteins were shown to contain transcriptional activation domains. These results suggest that transcriptional activation cascades involving ERF proteins may be important for plant defense to pathogen attack and that some ERF family members could be involved in the cross-talk between SA-and jasmonic acid-signaling pathways.