ANALYSIS OF REASSORTMENT AND SUPERINFECTION DURING MIXED INFECTION OF VERO CELLS WITH BLUETONGUE VIRUS SEROTYPE-10 AND SEROTYPE-17
ANALYSIS OF REASSORTMENT AND SUPERINFECTION DURING MIXED INFECTION OF VERO CELLS WITH BLUETONGUE VIRUS SEROTYPE-10 AND SEROTYPE-17
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DOI：
10.1099/0022-1317-70-10-2595
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发表时间：
1989-10-01
影响因子：
3.8
通讯作者：
BELLROBINSON, D
中科院分区：
文献类型：
--
作者：
RAMIG, RF;GARRISON, C;BELLROBINSON, D
研究了蓝舌病病毒（BTV）血清型10和17混合感染Vero细胞期间基因组片段的重配，使用非选择性条件分析混合感染的后代。发现重配是BTV复制周期中的早期事件，表明子代BTV基因组经历单轮重配。在等感染复数的杂交中观察到个体基因组片段的非随机分离，并在不等感染复数的杂交中得到证实。异步感染表明，如果第二个病毒在第一个病毒之后超过4小时，则重复感染排除导致重复感染病毒不能将基因组片段贡献给reflectants。这些结果的进化和流行病学的BTV的意义进行了讨论。
The reassortment of genome segments during mixed infection of Vero cells with bluetongue virus (BTV) serotypes 10 and 17 was investigated, using non-selective conditions for analysis of the progeny of mixed infections. Reassortment was found to be an early event in the BTV replication cycle, indicating that progeny BTV genomes undergo a single round of reassortment. Non-random segregation of individual genome segments was observed in crosses at equal multiplicity of infection, and was confirmed in crosses performed at unequal multiplicity. Asynchronous infections showed that superinfection exclusion resulted in the failure of the superinfecting virus to contribute genome segments to reassortants if the second virus followed the first by more than 4 h. The significance of these results for the evolution and epidemiology of BTV is discussed.