Molecular characterization of class I histone deacetylases and their expression in response to thermal and oxidative stresses in the red flour beetle, Tribolium castaneum
Molecular characterization of class I histone deacetylases and their expression in response to thermal and oxidative stresses in the red flour beetle, Tribolium castaneum
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赤拟谷盗 I 类组蛋白脱乙酰酶的分子特征及其响应热和氧化应激的表达
DOI：
10.1007/s10709-019-00065-3
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发表时间：
2019-08-01
期刊：
影响因子：
1.5
通讯作者：
Wang, Jianjun
中科院分区：
文献类型：
--
作者：
Chen, Minxuan;Zhang, Nan;Wang, Jianjun
核心组蛋白的可逆乙酰化在基因转录的表观遗传调控中起着重要作用，并且由组蛋白乙酰转移酶（HAT）和组蛋白去乙酰化酶（HDAC）的作用控制。虽然HDAC已经在果蝇中得到了很好的研究，但来自昆虫害虫的信息仍然有限。在本研究中，我们克隆和鉴定了赤拟谷盗Tribolium castaneum中的三种I类酶，TcHDAC 1，TcHDAC 3和TcHDAC 8。表达谱分析显示T. CastaneumHDAC基因在所有发育阶段和检查的组织中表达。从预蛹到1日龄蛹，三种T的mRNA表达水平均显著增加。castaneumHDAC基因。TcHDAC 1和TcHDAC 3在胸部的表达量最高，而TcHDAC 8在脂肪体中表达量最高。此外，T.发现栗HDAC基因对热、冷和氧化胁迫有响应。热胁迫处理降低了T. castaneumHDAC基因，其转录被百草枯处理诱导。这些结果提示I类HDAC基因在T.栗树发育和应激反应。
Reversible acetylation of core histones plays an important role in the epigenetic regulation of gene transcription, and is controlled by the action of histone acetyltransferases (HATs) and histone deacetylases (HDACs). While HDACs have been well studied inDrosophila melanogaster, information from insect pests is still limited. In the current study, we cloned and characterized three class I enzymes, TcHDAC1, TcHDAC 3 and TcHDAC 8, in the red flour beetle,Tribolium castaneum. Expression profiling showed thatT. castaneumHDAC genes are expressed in all developmental stages and tissues examined. A dramatic increase of mRNA expression level was observed from prepupae to 1-day-old pupae for all threeT. castaneumHDAC genes. BothTcHDAC1andTcHDAC3exhibited the highest mRNA expression levels in thorax, whereasTcHDAC8was highly expressed in fat body. Furthermore,T. castaneumHDAC genes were found to respond to heat, cold and oxidative stresses. While the heat-stress treatment decreased the mRNA expression levels ofT. castaneumHDAC genes, their transcripts were induced by paraquat treatment. These results suggest a possible role for class I HDAC genes in the epigenetic regulation ofT. castaneumdevelopment and stress responses.