MIGRATORY AND POSTMIGRATORY MOUSE PRIMORDIAL GERM-CELLS BEHAVE DIFFERENTLY IN CULTURE
MIGRATORY AND POSTMIGRATORY MOUSE PRIMORDIAL GERM-CELLS BEHAVE DIFFERENTLY IN CULTURE
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DOI：
10.1016/0092-8674(86)90005-x
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发表时间：
1986-03-28
期刊：
影响因子：
64.5
通讯作者：
WYLIE, CC
中科院分区：
文献类型：
--
作者：
DONOVAN, PJ;STOTT, D;WYLIE, CC
在所有的脊椎动物群体中，生殖系的祖先，原始生殖细胞（PGCs）在胚胎发育的早期出现在胚轴外，并转移到发育中的性腺。我们在体外研究了分离的性腺前和性腺PGCs在胚胎来源细胞系的饲喂层上的行为。经组织化学和血清学鉴定的性腺前生殖细胞在体外具有活跃的运动，并表现出侵袭性细胞的行为特征。然而，从发育性腺中分离的PGCs表现出很少的运动活性，并且在相同的细胞基质上不具有侵袭性。这些观察结果表明，PCGs在进入性腺原时经历了表型的重大变化。
In all vertebrate groups, the progenitors of the germ line, the primordial germ cells (PGCs) arise extragonadally and move to the developing gonad early in embryonic development. We have examined the behavior of isolated pregonadal and gonadal PGCs in vitro on feeder layers of an embryo-derived cell line. Histochemically and serologically identified pregonadal germ cells are found to be actively motile in vitro and, furthermore, show behavior characteristic of invasive cells. PGCs isolated from the developing gonad, however, show little locomotory activity and are not invasive on the same cellular substrate. These observations suggest that PCGs undergo a major change in phenotype at the time of their entry into the gonad anlagen.