Association between underexpression of microrna-203 and clinicopathological significance in hepatocellular carcinoma tissues.
Association between underexpression of microrna-203 and clinicopathological significance in hepatocellular carcinoma tissues.
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DOI：
10.1186/s12935-015-0214-0
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发表时间：
2015
影响因子：
5.8
通讯作者：
Chen G
中科院分区：
文献类型：
--
作者：
Liu Y;Ren F;Rong M;Luo Y;Dang Y;Chen G
尽管最近的研究已经显示了miR-203作为癌症相关生物标志物的效用，但miR-203在HCC中的经验证的临床意义仍然不清楚。本研究的目的是评估miR-203表达与HCC患者临床病理特征之间的关系。通过定量逆转录聚合酶链反应（qRT-PCR）评估95例福尔马林固定、石蜡包埋（FFPE）的HCC组织及其配对的相邻非癌组织中miR-203的表达。同时，分析miR-203的表达及其与各种临床病理参数和患者复发的相关性。miR-203在肝癌组织中的相对表达水平为1.1651 ± 0.70378，显著低于癌旁肝组织中的相对表达水平（2.2408 ± 0.75351，P < 0.001）。miR-203低表达诊断HCC的曲线下面积（AUC）为0.85（95% CI：0.796 ~ 0.904，P = 0.027），以miR-203在所有组织（包括HCC和正常肝组织）中的中位表达评估的截止值为1.99。miR-203的表达与肿瘤转移呈负相关（r =-0.254，P = 0.013），临床肿瘤淋巴结转移（TNM）分期（r =-0.300，P = 0.003），nm 23表达p21表达（r =-0.223，P = 0.030）、微血管密度（r =-0.206，P = 0.045）与肝硬化的发生呈正相关（r = 0.487，P < 0.001）。miR-203低表达组复发时间（57.949 ± 4.184）个月略长于高表达组（54.682 ± 2.591）个月，但差异无统计学意义（χ 2 = 0.206，P = 0.650）。MiR-203在HCC的发生、发展中起着重要作用，可作为HCC恶化的预测因子。miR-203可能成为肝癌分子治疗的新靶点。
Although recent studies have shown the utility of miR-203 as a cancer-relevant biomarker, the validated clinical significance of miR-203 in HCC remains obscure. The aim of the present study was to evaluate the relationship between miR-203 expression and clinicopathological features in HCC patients. MiR-203 expression in 95 formalin-fixed, paraffin embedded (FFPE) HCC tissues and their paired adjacent non-cancerous tissues was evaluated by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Simultaneously, expression of miR-203 and its correlation with a variety of clinicopathological parameters and patient recurrence was analyzed. The relative level of miR-203 was 1.1651 ± 0.70378 in HCC tissues, significantly lower than its expression in the corresponding adjacent non-cancerous liver tissues (2.2408 ± 0.75351, P < 0.001). The area under curve (AUC) of low miR-203 expression to diagnose HCC was 0.85 (95 % CI: 0.796 ~ 0.904, P = 0.027) at a cut-off value 1.99 evaluated by the median expression of miR-203 in all tissues, including HCC and normal liver tissues. Expression of miR-203 was negatively correlated to metastasis (r = −0.254, P = 0.013), clinical tumor nodes metastasis (TNM) stage (r = −0.300, P = 0.003), nm23 expression (r = −0.292, P = 0.004), p21 expression (r = −0.223, P = 0.030), microvessel density (MVD)(r = −0.206, P = 0.045) and was positively correlated to cirrhosis (r = 0.487, P < 0.001). Additionally, the recurrent time of lower miR-203 expression group was 57.949 ± 4.184 months, slightly longer than that in the high expression group (54.682 ± 2.591 months), however, no significant difference was noted (Chi-square = 0.206, P = 0.650). MiR-203 plays a vital role in the carcinogenesis and progression of HCC, which makes itself as a predictor for the deterioration of HCC. Furthermore, miR-203 may become a new target for molecular therapy in HCC.
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影响因子：
13.5
作者：
Roayaie, Sasan;Obeidat, Khaled;Sposito, Carlo;Mariani, Luigi;Bhoori, Sherrie;Pellegrinelli, Alessandro;Labow, Daniel;Llovet, Josep M.;Schwartz, Myron;Mazzaferro, Vincenzo
通讯作者：
Mazzaferro, Vincenzo
影响因子：
--
作者：
Zhu, Xiangyu;Er, Kejun;Shi, Hong
通讯作者：
Shi, Hong
基于肝细胞癌的肝血液动力学基于经导管动脉化学栓塞。
影响因子：
--
作者：
Murata S;Mine T;Ueda T;Nakazawa K;Onozawa S;Yasui D;Kumita S
通讯作者：
Kumita S
影响因子：
120.7
作者：
Schetter, Aaron J.;Leung, Suet Yi;Harris, Curtis C.
通讯作者：
Harris, Curtis C.
影响因子：
4.3
作者：
Viticchie, Giuditta;Lena, Anna Maria;Melino, Gerry
通讯作者：
Melino, Gerry