Effect of short-term hormone therapy on oxidative stress and endothelial function in African American and Caucasian postmenopausal women
Effect of short-term hormone therapy on oxidative stress and endothelial function in African American and Caucasian postmenopausal women
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DOI：
10.1016/s0015-0282(03)00153-5
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发表时间：
2003-05-01
影响因子：
6.7
通讯作者：
Ahokas, A
中科院分区：
文献类型：
--
作者：
Ke, RW;Pace, DT;Ahokas, A
目的：在绝经后妇女(PMW)中，评价短期雌激素/孕激素治疗对自由基氧化应激的影响。此外，还测定了羟色胺对血浆一氧化氮(NO)活性的影响，以此作为血管内皮功能的指标。我们调查了这些标志物与高血压的关系以及吸烟、糖尿病和高血压的心血管危险因素。设计：一项比较干预前后的前瞻性观察性研究。地点：学术研究中心。患者(S)：27名(14名非洲裔美国人和13名高加索人)PMW志愿者。干预(S)：连续6周的雌激素/孕激素联合治疗。主要结果衡量(S)：比较高血压治疗前后血浆游离8-表前列腺素F-2α(8-异前列腺素)浓度作为氧化应激的衡量标准。硝酸镉还原为亚硝酸盐后，用Greiss反应测定NO的稳定氧化代谢产物亚硝酸盐。结果：高压氧治疗6周后，血浆游离8-异前列腺素水平显著下降。虽然几乎所有受试者都受益于游离8-异前列腺素的减少，但至少有一个心血管危险因素(n=19)的PMW患者的游离8-异前列腺素水平高于没有危险因素的受试者。高压氧治疗6周后血浆亚硝酸盐水平升高，但差异无统计学意义。高加索人甲状旁腺素治疗6周后血浆亚硝酸盐水平较非裔美国人显著升高，而非裔美国人亚硝酸盐水平几乎没有变化。结论：短期服用羟色胺可显著降低甲状旁腺素患者的氧化应激反应，且在不同种族之间具有一致性。然而，在非裔美国人和高加索人的PMW中，观察到内皮细胞对高血压的无反应存在种族差异。(C)2003年，由美国生殖医学学会提供。
Objective: In postmenopausal women (PMW), the effect of a short-term course of estrogen/progestin HT on free radical oxidative stress was evaluated. In addition, HT's effect on plasma nitric oxide (NO) activity was determined as a measure of vascular endothelial function. We investigated the relationship of these markers and HT across race and the cardiovascular risk factors of smoking, diabetes and hypertension.Design: A prospective, observational study comparing preintervention and postintervention.Setting: Academic research center.Patient(s): Twenty-seven (14 African American and 13 Caucasian) PMW volunteers.Intervention (s): Six weeks of continuous, combined estrogen/progestin HT.Main Outcome Measure(s): Plasma concentrations of free 8-epi-prostaglandin F-2alpha (8-isoprostane) before and after HT were compared as a measure of oxidative stress. Nitrite, the stable oxidation metabolite of NO, was measured by the Greiss reaction after nitrate reduction to nitrite with cadmium.Results: Plasma levels of free 8-isoprostane decreased significantly after 6 weeks of HT. Although almost all subjects benefited from the reduction in free 8-isoprostane, PMW with at least one cardiovascular risk factor (n = 19) demonstrated higher free 8-isoprostane than did subjects with no risk factors. Plasma levels of nitrite increased after 6 weeks of HT, but the difference was not statistically significant. Caucasian PMW demonstrated a greater increase in plasma levels of nitrite after 6 weeks of HT as compared with African American subjects, who exhibited almost no change.Conclusion(s): Short-term administration of HT significantly reduces oxidative stress in PMW and is consistent across race. However, there was an observed racial difference in endothelial NO response to HT between African American and Caucasian PMW. (C) 2003 by American Society for Reproductive Medicine.