The isolation of polyadenylate with unreacted cellulose.
The isolation of polyadenylate with unreacted cellulose.
复制标题
聚腺苷酸与未反应的纤维素的分离。
DOI：
10.1016/0006-291x(72)90232-x
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发表时间：
1972
影响因子：
3.1
通讯作者：
H. Amos
中科院分区：
文献类型：
--
作者：
P. Kitos;G. Saxon;H. Amos
未与聚脱氧胸苷酸、聚尿苷酸或醋酸酯酯化的纤维素，在pH值为5.5或更低的高盐溶液或低盐溶液中选择性地结合聚腺苷酸（聚[A]）和含有聚[A]的rna。它不结合异聚RNA，但对DNA有一定的亲和力，特别是单链形式的DNA。这些结合特性可用于分离真核细胞的异质核和信使rna，用于分析和制备目的。
Cellulose, unesterified with polydeoxythymidylate, polyuridylate or acetate selectively binds polyadenylate (poly [A]) and poly [A]- containing RNAs in high salt solution or in low salt solution at pH values of 5.5 or less. It does not bind heteropolymeric RNA but does have some affinity for DNA, particularly in its single-stranded form. These binding properties are useful in the isolation of heterogeneous nuclear and messenger RNAs of eukaryotic cells for analytic and preparative purposes.