Pelagivirga sediminicola gen. nov., sp. nov. isolated from the Bohai Sea
Pelagivirga sediminicola gen. nov., sp. nov. isolated from the Bohai Sea
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Pelagivirga sediminicola gen.
DOI：
10.1099/ijsem.0.003015
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发表时间：
2018
影响因子：
2.8
通讯作者：
ZHao Huilin
中科院分区：
文献类型：
--
作者：
Ji Xiaofei;Zhang Cong;Zhang Xiying;Xu Zheng;Ding Yunfei;Zhang Yimei;Song Qing;Li Boqing;ZHao Huilin
对从渤海海洋沉积物中分离到的一株革兰氏阴性细菌BH-SD 19 T进行了多相分类研究。BH-SD 19 T细胞为无鞭毛、无滑动、椭圆形杆状，宽0.5-1.0 μm，长1.0-2.0 μm。BH-SD 19 T是严格好氧的，氧化酶和过氧化氢酶阳性。生长发生在15-40 °C（最佳35 °C）、pH 6.0-8.5（最佳7.0-7.5）和1- 10%（w/v）NaCl（最佳2%）下。 主要脂肪酸为C19：0环ω 8 c（46.5%）、C16：0（20.3%）和C18：1ω 7 c和/或C18：1ω6c（10.6%）。 主要的呼吸醌是Q-10。主要的极性脂质是磷脂酰甘油、磷脂酰乙醇胺和一种未鉴定的磷脂。DNA的G+C含量为64.0mol%。BH-SD 19 T与Pontibacaminivorans（95.2%）和Roseovarius（93.4- 95.2%）的16 SrRNA序列相似性最高。 BH-SD 19 T与其它近缘种的序列相似性均在95.0%以下。 基于16 S rRNA基因序列构建的系统发育树表明，BH-SD 19 T形成了一个独特的谱系，并且没有加入任何已知的属。表型、化学分类学和系统发育学数据表明BH-SD 19 T代表杜鹃花科中的一个新属和新种，命名为Pelagivirga sediminicolagen。十一月，sp. nov.的报道。模式菌株为BH-SD 19 T（=CCTCC AB 2017074 T =KCTC 62202 T）。
A Gram-stain-negative bacterium, strain BH-SD19T, that was isolated from a marine sediment sample collected from the Bohai Sea, was subjected to a polyphasic taxonomic study. Cells of BH-SD19Tare non-flagellated, non-gliding, oval-shaped rods, 0.5–1.0 µm wide and 1.0–2.0 µm long. BH-SD19Tis strictly aerobic, and oxidase- and catalase-positive. Growth occurs at 15–40 °C (optimum 35 °C), at pH 6.0–8.5 (optimum 7.0–7.5) and with 1–10 % (w/v) NaCl (optimum 2 %). The predominant fatty acids are C19 : 0cyclo ω8c(46.5 %), C16 : 0(20.3 %) and C18 : 1ω7cand/or C18 : 1ω6c(10.6 %). The major respiratory quinone is Q-10. The major polar lipids are phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid. The DNA G+C content is 64.0 mol%. BH-SD19Tshows the highest 16S rRNA sequence similarity toPontibaca methylaminivorans(95.2 %) and strains of species of the genusRoseovarius(93.4–95.2 %). Sequence similarity values between BH-SD19Tand other phylogenetically related species are all below 95.0 %. Phylogenetic trees based on 16S rRNA gene sequences indicate that BH-SD19Tforms a distinct lineage and does not join any known genera in the trees. Phenotypic, chemotaxonomic and phylogenetic data indicate that BH-SD19Trepresents a novel genus and species in the familyRhodobacteraceae, for which the namePelagivirga sediminicolagen. nov., sp. nov. is proposed. The type strain is BH-SD19T(=CCTCC AB 2017074T=KCTC 62202T).