Sequence of human liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
Sequence of human liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
复制标题
人肝 6-磷酸果糖-2-激酶/果糖-2,6-双磷酸酶的序列。
DOI：
10.1093/nar/18.12.3652
复制
发表时间：
1990
影响因子：
14.9
通讯作者：
Pilkis,SJ
中科院分区：
文献类型：
--
作者：
Lange,AJ;Pilkis,SJ
EMBL登录号X52638 6-磷酸果糖-2-激酶/果糖-2，6-二磷酸酶催化果糖-2，6-二磷酸的合成和水解，果糖-2，6-二磷酸是6-磷酸-1-激酶的有效正效应物和果糖-2，6-二磷酸酶的负效应物。这种代谢产物的水平控制着糖酵解和糖异生过程中的碳流（1）。Algaier和Uyeda（2）报道了人肝脏6-PF-2-K/Fru-2，6-P2酶的部分序列。我们在此报道了以大鼠cDNA（3）为探针（4）从人肝文库中分离的人肝6-PF-2-K/Fru-2，6-P2酶的全长cDNA的序列。核苷酸序列由1801个核苷酸组成，编码471个氨基酸的蛋白质。推导的氨基酸序列与大鼠肝酶有95%的相同性（3）。
EMBL accession no. X52638 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase catalyzes the synthesis and hydrolysis of fructose-2, 6-bisphosphate, a potent positive effector of 6-phospho-1-kinase and a negative effector of fructose-2, 6-bisphosphatase. Levels of this metabolite control the flow of carbon through glycolysis and gluconeogenesis (1). A partial sequence of human liver 6-PF-2-K/Fru-2, 6-P2ase has been reported by Algaier and Uyeda (2). We report here the sequence of the full coding length cDNA of human liver 6-PF-2-K/Fru-2, 6-P2ase isolated from a human liver library in lambda gtl 1 using the rat cDNA (3) as a probe (4). The nucleotide sequence consists of 1801 nucleotides coding for a 471 amino acid protein. The deduced amino acid sequence is 95% identical to the rat liver enzyme (3).