Unusually large von Willebrand factor multimers preferentially promote young sickle and nonsickle erythrocyte adhesion to endothelial cells.
Unusually large von Willebrand factor multimers preferentially promote young sickle and nonsickle erythrocyte adhesion to endothelial cells.
复制标题
异常大的冯维勒布兰德因子多聚体优先促进年轻镰状和非镰状红细胞与内皮细胞的粘附。
DOI：
10.1002/ajh.2830420308
复制
发表时间：
1993
影响因子：
12.8
通讯作者：
McIntire,LV
中科院分区：
文献类型：
--
作者：
Wick,TM;Moake,JL;Udden,MM;McIntire,LV
Sickle red blood cells (RBC) suspended with endothelial cell (EC)-derived unusually large (UL) von Willebrand factor (vWF) multimers, but not large plasma vWF forms, adhered to human venous EC under shear flow conditions. When sickle RBC were separated by density gradient centrifugation, fractions rich in less dense RBC were the most adhesive to EC in the presence of ULvWF. Incubation of sickle RBC with monoclonal antibodies against platelet surface receptors GPlb or GPIIb/llla, or with the integrin receptor agonist Arg-Gly-Asp-Ser (RGDS) decreased the ULvWF-mediated sickle RBC adhesion to EC 84%,> 99%, and 90%, respectively. When incubated with EC before the flow studies, anti-GPIb antibody and RGDS inhibited the ULvWF-mediated sickle RBC adhesion to EC.
