Radiation-induced muscle damage in rats after fractionated high-dose irradiation
Radiation-induced muscle damage in rats after fractionated high-dose irradiation
复制标题
DOI：
10.2307/3579788
复制
发表时间：
1998-05-01
影响因子：
3.4
通讯作者：
Lee, MS
中科院分区：
文献类型：
--
作者：
Hsu, HY;Chai, CY;Lee, MS
通过光学显微镜和透射电子显微镜观察活组织检查，评价了雄性Wistar大鼠腿部在受到高剂量辐射后的肌肉损伤。将约40只成年雄性Wistar大鼠的双腿暴露于来自直线加速器的80戈伊6 MV X射线，每2戈伊分40次照射。从照射后第一天开始到12个月的间隔，根据实验设计，通过在不同的日子处死大鼠来获得连续的肌肉活检。照射后的腓肠肌显示出一个渐进的结构变化，在曝光完成后立即开始。照射后肌肉组织的形态学改变主要表现为出血、淋巴细胞浸润和明显的血管破坏。即使在照射后12个月，肌纤维之间的胶原蛋白量也增加。从照射后第1天到1年，肌细胞线粒体的数量、大小和结构均发生了明显变化。即使在最终暴露后12个月，通过光学和透射电子显微镜观察到肌肉组织也没有恢复。(C)1998年，辐射研究会。
Muscle damage in the legs of male Wistar rats after exposure to high-dose radiation was evaluated by observations of biopsied tissue made with light and transmission electron microscopy. Both legs of about 40 mature male Wistar rats were exposed to 80 Gy 6 MV X rays from a linear accelerator given in 40 fractions of 2 Gy. At intervals starting from the first day to 12 months after irradiation, serial muscle biopsies were obtained by sacrificing the rats on different days according to the experimental design. The irradiated gastrocnemius muscle displayed a progressive structural change that began immediately after the completion of the exposures. Morphological changes in irradiated muscles were characterized by hemorrhage, lymphocytic infiltration and obvious vascular destruction. Increased amounts of collagen between the muscle fibers were present even at 12 months after irradiation. There were significant changes in the number, size and structure of mitochondria in the muscle cells from the first day until 1 year after irradiation. Even 12 months after the final exposure, no recovery of muscle tissue was seen by light and transmission electron microscopy. (C) 1998 by Radiation Research Society.