Acute Brain Trauma in Mice Followed By Longitudinal Two-photon Imaging
Acute Brain Trauma in Mice Followed By Longitudinal Two-photon Imaging
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DOI：
10.3791/51559
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发表时间：
2014-04-01
影响因子：
1.2
通讯作者：
Khiroug, Leonard
中科院分区：
文献类型：
--
作者：
Paveliev, Mikhail;Kislin, Mikhail;Khiroug, Leonard
尽管急性脑外伤通常是由不同事故中的头部损伤引起的，而且影响了很大一部分人口，但目前还没有有效的治疗方法。目前使用的动物模型的局限性阻碍了对病理机制的理解。多光子显微镜允许在生理和病理条件下纵向研究完整动物脑内的细胞和组织。在这里，我们描述了两种急性脑损伤模型，通过创伤后条件下脑细胞行为的双光子成像研究。用尖针损伤选定的脑区，在脑实质中造成宽度和深度可控的创伤。该方法采用立体定向针刺，可与同时用药相结合。我们认为这种方法可以作为一种先进的工具来研究哺乳动物大脑急性创伤的病理生理后果的细胞机制。在这个视频中，我们将急性脑损伤与两种准备相结合：颅窗和颅骨薄化。我们还讨论了创伤后脑再生多期成像的两种准备方法的优点和局限性。
Although acute brain trauma often results from head damage in different accidents and affects a substantial fraction of the population, there is no effective treatment for it yet. Limitations of currently used animal models impede understanding of the pathology mechanism. Multiphoton microscopy allows studying cells and tissues within intact animal brains longitudinally under physiological and pathological conditions. Here, we describe two models of acute brain injury studied by means of two-photon imaging of brain cell behavior under posttraumatic conditions. A selected brain region is injured with a sharp needle to produce a trauma of a controlled width and depth in the brain parenchyma. Our method uses stereotaxic prick with a syringe needle, which can be combined with simultaneous drug application. We propose that this method can be used as an advanced tool to study cellular mechanisms of pathophysiological consequences of acute trauma in mammalian brain in vivo. In this video, we combine acute brain injury with two preparations: cranial window and skull thinning. We also discuss advantages and limitations of both preparations for multisession imaging of brain regeneration after trauma.