Can titanium oxide nanotubes facilitate intracellular delivery by laser-assisted photoporation?
Can titanium oxide nanotubes facilitate intracellular delivery by laser-assisted photoporation?
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DOI：
10.1016/j.apsusc.2020.148815
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发表时间：
2021-03
影响因子：
6.7
通讯作者：
L. Mohan;Srabani Kar;Ren Hattori;Miho Ishii-Teshima;P. Bera;S. Roy;T. Santra;T. Shibata;M. Nagai
中科院分区：
文献类型：
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作者：
L. Mohan;Srabani Kar;Ren Hattori;Miho Ishii-Teshima;P. Bera;S. Roy;T. Santra;T. Shibata;M. Nagai
在本研究中，一个新开发的纳秒脉冲激光辅助的二氧化钛纳米管（TNT）的高效细胞内输送已经建立。已经验证了纳秒脉冲激光照射TNT后细胞内递送的可能性的概念证明。采用电化学阳极氧化技术，在不同的电压和时间下，在钛板上制备了TNT。广泛的X射线光电子能谱（XPS）研究证实了在TNT中存在不同浓度的不同氧化钛物质，例如TiO 2、TixOy（TiO/Ti 2 O3/Ti 3 O 5），其通过不同的阳极氧化电压和时间沿着少量的Ti金属（Ti 0）形成。低价氧化物的形成导致TNT中的氧缺陷。XPS分析表明，阳极氧化电压和时间可以改变碳纳米管表面氧缺陷的浓度。由于氧缺陷的形成，纳米管具有准金属和金属性质。纳米管的这些性质可能有助于纳秒脉冲激光照射后通过各种机制向细胞内递送。使用这种技术，我们成功地将碘化丙啶（PI）和葡聚糖递送到HeLa细胞（HeLa-人宫颈癌细胞）中，在15 V/2 h形成的纳米管上具有高转染效率和细胞活力。
In the present study, a newly developed nanosecond pulse laser-assisted photoporation using titanium-oxide nanotubes (TNT) for highly efficient intracellular delivery has been established. The proof of concept for the possibilities of intracellular delivery after irradiation of nanosecond pulse laser on TNT has been validated. TNT on titanium sheets using the electrochemical anodization technique at different voltage and time has been developed. The extensive X-ray photoelectron spectroscopy (XPS) study confirms the presence of different titanium oxide species such as TiO2, TixOy(TiO/Ti2O3/Ti3O5) having different concentrations in TNT formed by different anodization voltage and time along with a minor quantity of Ti metal (Ti0). Formation of sub-oxides results in oxygen defects in TNT. It has also been evidenced from XPS that the anodization voltage and time can change the concentration of oxygen defects on the nanotubes. Due to the formation of oxygen defects, nanotubes have the quasi-metallic and metallic properties. These properties of the nanotubes may facilitate the intracellular delivery by various mechanisms after irradiation of nanosecond pulse laser. Using this technique, we successfully have delivered Propidium iodide (PI) and dextran into HeLa cells (HeLa- human cervical cancer cells) with high transfection efficiency and cell viability on nanotubes formed at 15 V/2 h.