Simultaneous degradation of the pesticides methyl parathion and chlorpyrifos by an isolated bacterial consortium from a contaminated site
Simultaneous degradation of the pesticides methyl parathion and chlorpyrifos by an isolated bacterial consortium from a contaminated site
复制标题
DOI：
10.1016/j.ibiod.2011.06.001
复制
发表时间：
2011-09-01
影响因子：
4.8
通讯作者：
Penuela, Gustavo
中科院分区：
文献类型：
--
作者：
Pino, Nancy;Penuela, Gustavo
使用从摩拉维亚（哥伦比亚麦德林）高度污染土壤中选择性富集获得的细菌联合体，对农药甲基异丙磷和毒死蜱的同时降解进行了测试。在该财团中鉴定的微生物是不动杆菌属、恶臭假单胞菌、芽孢杆菌属、铜绿假单胞菌、弗氏柠檬酸杆菌、寡养单胞菌属、黄杆菌属、普通变形杆菌、假单胞菌属、不动杆菌属、克雷伯氏菌属和变形杆菌属。在富含每种农药的培养基中，该财团能够在120小时内降解150 mg/l的甲基双硫磷和毒死蜱。当使用150毫克升（-1）的两种农药的混合物的百分比下降到72%的甲基敌百虫和39%的毒死蜱。随着葡萄糖添加到培养基中，该财团同时降解150毫克升（-1）的混合物中的农药。在土壤中进行了4种处理，包括添加葡萄糖和微生物、添加甘蔗和微生物、不添加营养物和不添加任何物品的微生物。在使用葡萄糖的处理中，120 h内甲基对硫磷和毒死蜱的降解率分别为98%和97%。用费氏弧菌监测，这种处理也实现了毒性降低的最高百分比。(C)2011爱思唯尔有限公司版权所有。
The simultaneous degradation of the pesticide methyl parathion and chlorpyrifos was tested using a bacterial consortium obtained by selective enrichment from highly contaminated soils in Moravia (Medellin, Colombia). Microorganisms identified in the consortium were Acinetobacter sp, Pseudomonas putida, Bacillus sp, Pseudomonas aeruginosa, Citrobacter freundii, Stenotrophomonas sp, Flavobacterium sp, Proteus vulgaris, Pseudomonas sp, Acinetobacter sp. Klebsiella sp and Proteus sp. In culture medium enriched with each of the pesticides, the consortium was able to degrade 150 mg l(-1) of methyl parathion and chlorpyrifos in 120 h. When a mixture of 150 mg l(-1) of both pesticides was used the percentage decreased to 72% for methyl parathion and 39% for chlorpyrifos. With the addition of glucose to the culture medium, the consortium simultaneously degraded 150 mg l(-1) of the pesticides in the mixture. 4 treatments were carried out in soil that included the addition of glucose with microorganisms, the addition of sugar cane with microorganisms, microorganisms without nutrient addition and without the addition of any item. In the treatment in which glucose was used, degradation percentages of methyl parathion and chlorpyrifos of 98% and 97% respectively were obtained in 120 h. This treatment also achieved the highest percentage of reduction in toxicity, monitored with Vibrio fischeri. (C) 2011 Elsevier Ltd. All rights reserved.