Candida albicans stimulates Streptococcus mutans microcolony development via cross-kingdom biofilm-derived metabolites.
Candida albicans stimulates Streptococcus mutans microcolony development via cross-kingdom biofilm-derived metabolites.
复制标题
白色念珠菌通过跨王室生物膜衍生的代谢产物刺激链球菌突变体的微殖民发育。
DOI：
10.1038/srep41332
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发表时间：
2017-01-30
影响因子：
4.6
通讯作者：
Koo H
中科院分区：
文献类型：
--
作者：
Kim D;Sengupta A;Niepa TH;Lee BH;Weljie A;Freitas-Blanco VS;Murata RM;Stebe KJ;Lee D;Koo H
白色念珠菌是经常检测到的严重感染的变形链球菌菌斑生物膜的儿童影响与幼儿龋齿，一种流行的和昂贵的口腔疾病。C. albicans增强了S.变形杆菌在生物膜内生长，但与细菌积累相关的化学相互作用仍不清楚。因此，本研究旨在探讨这种跨界结合的微生物产物如何调节S。变形菌在生物膜中聚集。结果表明，细菌-真菌条件培养基（BF-CM）能显著促进S.变形菌和改变生物膜三维结构的剂量依赖性方式，导致扩大和密集包装的细菌细胞簇（小菌落）。有趣的是，BF-CM诱导S.变异株gtfBC表达（负责Gtf外切酶的产生），增强小菌落发育所必需的Gtf活性。使用最近开发的纳米培养系统，数据表明同时微菌落生长和gtfB激活原位BF-CM。BF-CM的进一步代谢物/色谱分析显示甲酸盐的量升高，并且存在通常已知表现出抗菌活性的拟南芥衍生的法尼醇。出乎意料的是，在检测到的水平（25-50 μM），法尼醇增强S。突变体-生物膜细胞生长、小菌落发育和类似于BF-CM生物活性的Gtf活性。总而言之，这些数据为跨王国相互作用的胞外微生物产物如何刺激细菌病原体在生物膜内的积累提供了新的见解。
Candida albicans is frequently detected with heavy infection of Streptococcus mutans in plaque-biofilms from children affected with early-childhood caries, a prevalent and costly oral disease. The presence of C. albicans enhances S. mutans growth within biofilms, yet the chemical interactions associated with bacterial accumulation remain unclear. Thus, this study was conducted to investigate how microbial products from this cross-kingdom association modulate S. mutans build-up in biofilms. Our data revealed that bacterial-fungal derived conditioned medium (BF-CM) significantly increased the growth of S. mutans and altered biofilm 3D-architecture in a dose-dependent manner, resulting in enlarged and densely packed bacterial cell-clusters (microcolonies). Intriguingly, BF-CM induced S. mutans gtfBC expression (responsible for Gtf exoenzymes production), enhancing Gtf activity essential for microcolony development. Using a recently developed nanoculture system, the data demonstrated simultaneous microcolony growth and gtfB activation in situ by BF-CM. Further metabolites/chromatographic analyses of BF-CM revealed elevated amounts of formate and the presence of Candida-derived farnesol, which is commonly known to exhibit antibacterial activity. Unexpectedly, at the levels detected (25–50 μM), farnesol enhanced S. mutans-biofilm cell growth, microcolony development, and Gtf activity akin to BF-CM bioactivity. Altogether, the data provide new insights on how extracellular microbial products from cross-kingdom interactions stimulate the accumulation of a bacterial pathogen within biofilms.