EFFICIENT INDUCTION BY AMIPROPHOS-METHYL AND FLOW-CYTOMETRIC SORTING OF MICRONUCLEI IN NICOTIANA-PLUMBAGINIFOLIA
EFFICIENT INDUCTION BY AMIPROPHOS-METHYL AND FLOW-CYTOMETRIC SORTING OF MICRONUCLEI IN NICOTIANA-PLUMBAGINIFOLIA
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DOI：
10.1007/bf00393862
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发表时间：
1987-12-01
期刊：
影响因子：
4.3
通讯作者：
DIJKHUIS, P
中科院分区：
文献类型：
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作者：
DELAAT, AMM;VERHOEVEN, HA;DIJKHUIS, P
甲基胺草磷（APM）是一种潜在的除草剂，作用于微管水平。用该试剂处理烟草悬浮细胞，可观察到高度的中期阻滞，单个染色体和成组染色体分散在整个细胞中，为细胞学和染色体分离提供了良好的应用前景。在长期暴露于药物后，染色体解凝聚并形成微核。根据其DNA含量，通过流式细胞术分选微核。对微核技术在部分基因组转移和基因定位中的应用前景进行了展望。
Amiprophos-methyl (APM) is a potential herbicide which acts at the level of microtubules. By exposure of suspension cells ofNicotiana plumbaginifoliato this agent, a high degree of metaphase arrest was observed and single as well as groups of chromosomes were scattered throughout the cell, offering good prospects for application in cytology and chromosome isolation. After prolonged exposure to the drug, the chromosomes decondensed and micronuclei were formed. Based on their DNA content, the micronuclei were sorted by flow cytometry. Prospects for application of isolated micronuclei for partial genome transfer and gene mapping are discussed.