Rapid Introgression of the Fusarium Wilt Resistance Gene into an Elite Cabbage Line through the Combined Application of a Microspore Culture, Genome Background Analysis, and Disease Resistance-Specific Marker Assisted Foreground Selection.
Rapid Introgression of the Fusarium Wilt Resistance Gene into an Elite Cabbage Line through the Combined Application of a Microspore Culture, Genome Background Analysis, and Disease Resistance-Specific Marker Assisted Foreground Selection.
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DOI：
10.3389/fpls.2017.00354
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发表时间：
2017
影响因子：
5.6
通讯作者：
Lv H
中科院分区：
文献类型：
--
作者：
Liu X;Han F;Kong C;Fang Z;Yang L;Zhang Y;Zhuang M;Liu Y;Li Z;Lv H
白菜是世界上重要的经济蔬菜。白菜枯萎病（CFW）是一种对油菜造成严重产量和品质损失的破坏性病害。利用抗CFW品种是缓解CFW影响的最有效策略。01-20是一个性状优良、配合力高的优质白菜品系，但对CFW非常敏感。为了快速将CFW抗性基因转移到01-20株植物中，我们利用小孢子培养从01-20（高易感）和96-100（高抗性）的杂交中培养出230个双单倍体（DH）系。其中一个DH系（即D134）对CFW具有高度抗性，其表型表现与01-20相似。因此，选用D134作为抗性供体亲本。利用01 ~ 20系和96 ~ 100系的全基因组重测序数据，生成24个插入缺失标记，分析回交后代的基因组背景。以CFW抗性基因FOC1为基础，开发了SSR标记Frg13，用于前景选择。我们用这些标记筛选了240个BC1和280个BC2个体，并评估了他们的表型表现。最佳个体BC1和BC2的复发亲本基因组（PRPG）比例分别为95.8和99.1%。最后，从80个BC2F1个体中鉴定出一个最佳个体，命名为YR01-20，其FOC1等位基因纯合，基因组背景和表型与01-20基本相同。本研究结果可为小孢子培养、全基因组背景分析和抗病特异性标记选择的结合应用提供一种快速有效的改良优良系的方法。此外，本研究开发的白菜系为选育抗cfw的白菜新品种提供了优良材料。
Cabbage is an economically important vegetable worldwide. Cabbage Fusarium Wilt (CFW) is a destructive disease that results in considerable yield and quality losses in cole crops. The use of CFW-resistant varieties is the most effective strategy to mitigate the effects of CFW. 01-20 is an elite cabbage line with desirable traits and a high combining ability, but it is highly susceptible to CFW. To rapidly transfer a CFW resistance gene into 01-20 plants, we used microspore cultures to develop 230 doubled haploid (DH) lines from a cross between 01-20 (highly susceptible) and 96-100 (highly resistant). One of the generated DH lines (i.e., D134) was highly resistant to CFW and exhibited a phenotypic performance that was similar to that of line 01-20. Therefore, D134 was applied as the resistance donor parent. We generated 24 insertion–deletion markers using whole genome resequencing data for lines 01-20 and 96-100 to analyze the genomic backgrounds of backcross (BC) progenies. Based on the CFW resistance gene FOC1, a simple sequence repeat (SSR) marker (i.e., Frg13) was developed for foreground selections. We screened 240 BC1 individuals and 280 BC2 individuals with these markers and assessed their phenotypic performance. The proportion of recurrent parent genome (PRPG) of the best individuals in BC1 and BC2 were 95.8 and 99.1%. Finally, a best individual designated as YR01-20 was identified from 80 BC2F1 individuals, with homozygous FOC1 allele and genomic background and phenotype almost the same as those of 01-20. Our results may provide a rapid and efficient way of improving elite lines through the combined application of microspore culture, whole-genome background analysis, and disease resistance-specific marker selection. Additionally, the cabbage lines developed in this study represent elite materials useful for the breeding of new CFW-resistant cabbage varieties.