Knockdown of two trehalose-6-phosphate synthases severely affects chitin metabolism gene expression in the brown planthopper Nilaparvata lugens
Knockdown of two trehalose-6-phosphate synthases severely affects chitin metabolism gene expression in the brown planthopper Nilaparvata lugens
复制标题
两种海藻糖-6-磷酸合酶的敲低严重影响褐飞虱中几丁质代谢基因的表达
DOI：
10.1002/ps.4287
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发表时间：
2017
影响因子：
4.1
通讯作者：
Bin Tang
中科院分区：
文献类型：
--
作者：
Mengmeng Yang;Lina Zhao;Qida Shen;Guoqiang Xie;Shigui Wang;Bin Tang
背景drna干扰与数字基因表达（DGE）分析相结合可用于研究基因功能。海藻糖- 6 -磷酸合成酶（TPS）在海藻糖的合成和昆虫的发育中起着关键作用。结果RNAi敲低NlTPS1和NlTPS2 48 h后，9个和4个几丁质酶基因的表达均显著降低。此外，发现表型异常，约30%的昆虫死亡。NlTPS1和NlTPS2敲除72 h后，hkandg6pi2的表达显著降低，而gfat、gnpna和uap3的表达显著升高。pgm1表达在TPS2敲除后显著降低，而pgm2表达在TPS1敲除48 h后显著升高，3个echs基因表达均降低。NlTPS1和NlTPS2敲除后48 h，所有12个几丁质降解基因的mRNA表达量均下降，cht2、Cht3、Cht6、Cht7、cht10和engasmrna表达量在NlTPS1和NlTPS2敲除后72 h仍显著下降。结论tps2基因的沉默可能会导致几丁质代谢相关基因的失调，从而导致蜕皮畸形和死亡率的增加，是今后害虫防治的潜在靶点。©2016化学工业协会
BACKGROUNDRNA interference combined with digital gene expression (DGE) analysis can be used to study gene function. Trehalose‐6‐phosphate synthase (TPS) plays a key role in the synthesis of trehalose and insect development.RESULTSDGE analysis revealed that the expression of nine or four chitinase genes was reduced significantly 48 h after NlTPS1 and NlTPS2 knockdown by RNAi, respectively. Additionally, abnormal phenotypes were noted, and approximately 30% of insects died.HKandG6PI2expression decreased significantly whereasGFAT,GNPNAandUAPexpression increased significantly 72 h after NlTPS1 and NlTPS2 knockdown.PGM1expression decreased significantly after TPS2 knockdown, whereasPGM2expression increased significantly and the expression of threeCHSgenes decreased 48 h after TPS1 knockdown. The mRNA expression of all 12 chitin degradation genes decreased 48 h after NlTPS1 and NlTPS2 treatment, andCht2,Cht3,Cht6,Cht7,Cht10andENGaselevels remained significantly decreased up to 72 h after NlTPS1 and NlTPS2 knockdown.CONCLUSIONSThese results demonstrate that silencing ofTPSgenes can lead to increased moulting deformities and mortality rates owing to the misregulation of genes involved in chitin metabolism, andTPSgenes are potential pest control targets in the future. © 2016 Society of Chemical Industry