Single-cell transcriptional analysis reveals developmental stage-dependent changes in retinal progenitors in the murine early optic vesicle
Single-cell transcriptional analysis reveals developmental stage-dependent changes in retinal progenitors in the murine early optic vesicle
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单细胞转录分析揭示了小鼠早期视泡视网膜祖细胞的发育阶段依赖性变化
DOI：
10.1016/j.bbrc.2021.01.043
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Kanai Y.
中科院分区：
文献类型：
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作者：
Yamada R;Oguri A;Fujiki K;Shirahige K;Takezoe H;Takahashi N;Kanai Y.
发育中的胚胎眼中的视神经泡含有大量的神经上皮祖细胞，这些祖细胞随后分化成功能不同的视杯区域，例如神经视网膜、色素上皮和视柄。为了研究视杯区域化之前早期视泡的细胞类型多样性，我们使用来自小鼠胚胎假定眼部区域的 7989 个细胞进行了单细胞 RNA 测序 (scRNA-seq)，这些细胞处于 12-26 体节阶段的五个发育时间点。我们证明了七个视神经泡群体的存在。此外，这四个视网膜祖细胞群可以根据其阶段依赖性时间点进行分类，并且这些细胞在视杯区域化之前表现出几个结构和代谢关键基因的表达改变，例如Col9a1和Ckb。根据这些数据，我们以单细胞分辨率提供了关于初始视网膜规范期间阶段依赖性转录谱的第一份报告，并强调了小鼠视泡结构的意外发育异质性。
The optic vesicle in the developing embryonic eye contains a multitude of neuroepithelial progenitors that subsequently differentiate into functionally distinct domains of the optic cup, such as the neural retina, pigment epithelium, and optic stalk. To investigate cell-type diversity across early optic vesicles before regionalization of the optic cup, we performed single-cell RNA-sequencing (scRNA-seq) using 7989 cells from the presumptive eye area in mouse embryos at the 12–26-somite stages at five developmental time points. We demonstrated the presence of seven optic vesicle populations. Moreover, the four populations of retinal progenitor cells could be classified according to their stage-dependent time point, and these cells exhibited altered expression of several structural and metabolic key genes, such asCol9a1andCkb, just before regionalization of the optic cup. From these data, we provide the first report on stage-dependent transcriptional profiles during initial retinal specification at single-cell resolution and highlight the unexpected developmental heterogeneity of the murine optic vesicle structure.