Identification of a new glutathione S-transferase from rat liver cytosol.
Identification of a new glutathione S-transferase from rat liver cytosol.
复制标题
从大鼠肝细胞质中鉴定出一种新的谷胱甘肽 S-转移酶。
DOI：
10.1016/0006-291x(84)90778-2
复制
发表时间：
1984
影响因子：
3.1
通讯作者：
Tu,CP
中科院分区：
文献类型：
--
作者：
Reddy,CC;Li,N;Tu,CP
从Sprague-Dawley大鼠肝脏匀浆的105,000× g上清中纯化出一种新的谷胱甘肽s -转移酶。纯化酶的比活性分别为1.8和0.12 μmol。分钟−1。Mg - 1分别对1-氯2,4 -二硝基苯和过氧化氢异丙苯的反应。亚基组成的SDS凝胶电泳数据显示，新转移酶由两个亚基组成，其mrr相同，为24,400 （Y α家族）。我们对大鼠肝脏poly (A) rna的体外翻译实验和底物特异性数据表明，该亚基不同于先前报道的大鼠肝脏谷胱甘肽s转移酶的Ya， Yb和Yc亚基。该同工酶对1,2环氧-3-（对硝基苯氧基）丙烷、乙酸和对硝基苯乙酸的活性分别为0.4、0.34和0.18 μ mol。分钟−1。Mg−1。
A new glutathione S-transferase has been purified to homogeneity from 105,000× g supernatant of Sprague-Dawley rat liver homogenates. The purified enzyme exhibited specific activities of approximately 1.8, and 0.12 μmoles. min− 1. mg− 1 toward 1-chloro 2, 4-dinitrobenzene and cumene hydroperoxide respectively. The SDS gel electrophoresis data on subunit composition revealed that the new transferase is composed of two subunits with an identical M r of 24,400 (Y α Family). Our in vitro translation experiments with rat liver poly (A) RNAs and substrate specificity data suggest that this subunit is different from the previously reported Ya, Yb and Yc subunits of rat liver glutathione S-transferases. Comparatively, the new isozyme showed significant activity toward 1, 2 epoxy-3-(P-nitrophenoxy)-propane, ethacrynic acid and P-nitrophenyl acetate, 0.4, 0.34 and 0.18 μ moles. min− 1. mg− 1 respectively.