Circadian rhythm affects the magnitude of contact hypersensitivity response in mice
Circadian rhythm affects the magnitude of contact hypersensitivity response in mice
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昼夜节律影响小鼠接触超敏反应的程度
DOI：
10.1111/all.15314
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发表时间：
2022
期刊：
影响因子：
12.4
通讯作者：
Kabashima Kenji
中科院分区：
文献类型：
--
作者：
Miyake Toshiya;Egawa Gyohei;Chow Zachary;Asahina Ryota;Otsuka Masayuki;Nakajima Saeko;Nomura Takashi;Shibuya Rintaro;Ishida Yoshihiro;Nakamizo Satoshi;Murata Teruasa;Kitoh Akihiko;Kabashima Kenji
BackgroundThe昼夜节律控制多种生物过程，包括免疫反应;然而，其对皮肤适应性免疫应答的影响仍不清楚。MethodsWe使用了一个完善的皮肤IV型过敏模型，接触性超敏反应（CHS）。我们用二硝基氟苯（DNFB）诱导CHS。小鼠致敏，并引发与DNFB在白天或在night.ResultsIn小鼠，一个积极的动物，我们发现，耳肿胀增加时，小鼠在夜间致敏相比，在白天。此外，在夜间致敏时，引流淋巴结（LN）中的细胞增殖和细胞因子产生得到促进。我们假设这些差异是由于体内白细胞分布的振荡通过肾上腺素能激素的昼夜生产。白天给予β2-肾上腺素能受体（β2AR）激动剂沙丁胺醇可减少血液中的免疫细胞数量，增加LN中的免疫细胞数量。与此相反，β 2 AR拮抗剂ICI 18551在夜间给药增加了血液中的免疫细胞数量，减少了LN中的免疫细胞数量。因此，严重程度的CHS反应加剧沙丁胺醇管理在白天和衰减ICI 18551管理在night.ConclusionOur研究表明，适应性CHS反应的幅度取决于昼夜节律，这方面的知识可能会提高人类过敏性接触性皮炎（ACD）的管理。
BackgroundThe circadian rhythm controls multiple biological processes, including immune responses; however, its impact on cutaneous adaptive immune response remains unclear.MethodsWe used a well‐established cutaneous type IV allergy model, contact hypersensitivity (CHS). We induced CHS using dinitrofluorobenzene (DNFB). Mice were sensitized and elicited with DNFB in the daytime or at night.ResultsIn mice, a nocturnally active animal, we found that ear swelling increased when mice were sensitized at night compared with in the daytime. In addition, cell proliferation and cytokine production in the draining lymph nodes (LNs) were promoted when sensitized at night. We hypothesized that these differences were due to the oscillation of leukocyte distribution in the body through the circadian production of adrenergic hormones. Administration of a β2‐adrenergic receptor (β2AR) agonist salbutamol in the daytime decreased the number of immune cells in blood and increased the number of immune cells in LNs. In contrast, a β2AR antagonist ICI18551 administration at night increased the number of immune cells in blood and decreased the number of immune cells in LNs. Accordingly, the severity of CHS response was exacerbated by salbutamol administration in the daytime and attenuated by ICI18551 administration at night.ConclusionOur study demonstrated that the magnitude of adaptive CHS response depends on the circadian rhythm and this knowledge may improve the management of allergic contact dermatitis (ACD) in humans.