Multiple Autoantibodies Display Association with Lymphopenia, Proteinuria, and Cellular Casts in a Large, Ethnically Diverse SLE Patient Cohort.
Multiple Autoantibodies Display Association with Lymphopenia, Proteinuria, and Cellular Casts in a Large, Ethnically Diverse SLE Patient Cohort.
复制标题
DOI：
10.1155/2012/819634
复制
发表时间：
2012
影响因子：
4
通讯作者：
James JA
中科院分区：
文献类型：
--
作者：
Lu R;Robertson JM;Bruner BF;Guthridge JM;Neas BR;Nath SK;Kelly JA;Moser Sivils KL;Chakravarty EF;Kamen DL;Gilkeson GS;Wallace DJ;Weisman MH;Scofield RH;Harley JB;James JA
目的。本研究评估高通量自身抗体筛查，并确定大型狼疮队列中相关的系统性红斑狼疮 (SLE) 临床特征。方法。在获得适当的知情同意后，从每位 SLE 研究参与者处获得临床和人口统计信息以及血清样本。筛选血清样本中 10 种不同的 SLE 自身抗体特异性，并使用条件逻辑回归分析检查其与 SLE ACR 标准和亚标准的关联。结果。在欧美SLE患者中，淋巴细胞减少患者中抗52kD Ro和RNP 68的自身抗体独立富集，颧骨皮疹患者中抗La和抗核糖体P增加，蛋白尿患者中抗dsDNA和抗Sm富集。在非裔美国 SLE 患者中，细胞管型与针对 dsDNA、Sm 和 Sm/nRNP 的自身抗体相关。结论。使用高通量、基于微珠的自身抗体检测方法，如前所述，具有 SLE ACR 肾脏标准的患者中的抗 dsDNA 显着富集。然而，淋巴细胞减少症与几种不同的自身抗体特异性有关。这些发现提供了有意义的信息，使临床医生和临床研究人员能够使用这种在临床应用中不断扩展的新方法来了解哪些自身抗体与常见种族群体中特定的 SLE 临床表现相关。
Purpose. This study evaluates high-throughput autoantibody screening and determines associated systemic lupus erythematosus (SLE) clinical features in a large lupus cohort. Methods. Clinical and demographic information, along with serum samples, were obtained from each SLE study participant after appropriate informed consent. Serum samples were screened for 10 distinct SLE autoantibody specificities and examined for association with SLE ACR criteria and subcriteria using conditional logistic regression analysis. Results. In European-American SLE patients, autoantibodies against 52 kD Ro and RNP 68 are independently enriched in patients with lymphopenia, anti-La, and anti-ribosomal P are increased in patients with malar rash, and anti-dsDNA and anti-Sm are enriched in patients with proteinuria. In African-American SLE patients, cellular casts associate with autoantibodies against dsDNA, Sm, and Sm/nRNP. Conclusion. Using a high-throughput, bead-based method of autoantibody detection, anti-dsDNA is significantly enriched in patienets with SLE ACR renal criteria as has been previously described. However, lymphopenia is associated with several distinct autoantibody specificities. These findings offer meaningful information to allow clinicians and clinical investigators to understand which autoantibodies correlate with select SLE clinical manifestations across common racial groups using this novel methodology which is expanding in clinical use.